Use of the confocal microscope to determine polyomavirus recombinant capsid-like particle entry into mouse 3T6 cells

The structural protein genes of polyomavirus were expressed in the baculovirus system, and the proteins were found to assemble into capsid-like particles capable of packaging insect cell DNA. Recombinant capsid-like particles could be produced that were composed of the various structural proteins (V...

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Bibliographic Details
Published in:Journal of virological methods 2000-02, Vol.84 (2), p.153-159
Main Authors: An, K, Lovgren, T.R, Tilley, M.B, Consigli, R.A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Capsid - genetics
Capsid - metabolism
Cell entry
Cell Line
Confocal microscopy
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Mice
Microbiology
Microscopy, Confocal - methods
Polyomavirus
Polyomavirus - genetics
Polyomavirus - pathogenicity
Polyomavirus - physiology
Recombinant capsid-like particles
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Techniques used in virology
Viral Structural Proteins - metabolism
Virion - genetics
Virion - metabolism
Virology
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Summary:The structural protein genes of polyomavirus were expressed in the baculovirus system, and the proteins were found to assemble into capsid-like particles capable of packaging insect cell DNA. Recombinant capsid-like particles could be produced that were composed of the various structural proteins (VP1, VP1/2, VP1/3 and VP1/2+VP3). Laser scanning confocal microscopy was used to determine if the various capsid-like particles could infect (enter) mouse 3T6 cells. Each of the various capsid-like particles was equally capable of cell entry as determined by indirect immunofluorescence confocal microscopy.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(99)00137-8