Abrogation of Oral Tolerance by Feeding Encapsulated Antigen

Results from previous studies have indicated that suppression of immune responses cannot be abrogated once oral tolerance has been established. Using a new methodology, OVA was encapsulated and fed to tolerized BDF1 mice. Results from these studies indicated that although IgG2a titers remained low,...

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Bibliographic Details
Published in:Cellular immunology 2000-02, Vol.199 (2), p.65-72
Main Authors: Barone, K.Siobhan, Reilly, Melinda R., Flanagan, Michael P., Michael, J.Gabriel
Format: Article
Language:English
Subjects:
Acrylic Resins - administration & dosage
Administration, Oral
Animals
Antigens - administration & dosage
Antigens - immunology
Capsules
Chickens
Female
Immune Tolerance - immunology
Immunoglobulin G - biosynthesis
Immunoglobulin G - blood
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Microspheres
ovalbumin
Ovalbumin - administration & dosage
Ovalbumin - immunology
Polymethacrylic Acids
Solubility
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Summary:Results from previous studies have indicated that suppression of immune responses cannot be abrogated once oral tolerance has been established. Using a new methodology, OVA was encapsulated and fed to tolerized BDF1 mice. Results from these studies indicated that although IgG2a titers remained low, total IgG and IgG1 antibody titers were no longer suppressed compared to controls. Furthermore, in vitro splenocyte proliferation was not significantly suppressed in tolerized mice fed encapsulated OVA. To determine whether oral tolerance could be abrogated in other strains of mice, BALB/c mice were tolerized and fed encapsulated OVA. Results from these studies indicated that IgG2a as well as IgG1 and total anti-OVA antibody titers were no longer suppressed. Although splenocyte proliferation did remain significantly suppressed in these mice, IFN-γ and IL-4 levels were no longer decreased. To the best of our knowledge, this is the first time that abrogation of an established oral tolerance has been demonstrated.
ISSN:0008-8749
1090-2163
DOI:10.1006/cimm.1999.1603