HSP70 Induction in the Brain Following Ethanol Administration in the Rat: Regulation by Glutathione Redox State

Changes in glutathione (GSH) and glutathione disulfide (GSSG) levels and/or redox status have been suggested to mediate the induction of heat shock proteins (HSPs) that follows exposure to oxidizing agents such as ethanol. Here we report the effects of ethanol administration to rats at intracellular...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-03, Vol.269 (2), p.397-400
Main Authors: Calabrese, Vittorio, Testa, Giuseppe, Ravagna, Agrippino, Bates, Timothy Edward, Giuffrida Stella, Anna Maria
Format: Article
Language:English
Subjects:
Animals
Brain - drug effects
Brain - metabolism
Ethanol - pharmacology
Glutathione - metabolism
HSP70 Heat-Shock Proteins - biosynthesis
Male
Oxidation-Reduction
Rats
Rats, Wistar
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Summary:Changes in glutathione (GSH) and glutathione disulfide (GSSG) levels and/or redox status have been suggested to mediate the induction of heat shock proteins (HSPs) that follows exposure to oxidizing agents such as ethanol. Here we report the effects of ethanol administration to rats at intracellular levels of GSH, GSSG, HSP70, and protein carbonyls in brain and liver. Following 7 days of ethanol administration, there was a significant decrease in GSH, a significant induction of HSP70, and a significant increase in protein carbonyls in all brain regions studied and in liver. In cortex, striatum, and hippocampus there was a significant correlation between (a) the decrease in GSH, (b) the increase in GSSG, and (c) the decrease in GSH/GSSG ratio and HSP70 levels induced in response to ethanol. These data support the hypothesis that a redox mechanism may be involved in the heat-shock signal pathway responsible for HSP70 induction in the brain.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.2311