Simultaneous quantification of retinol, retinal, and retinoic acid isomers by high-performance liquid chromatography with a simple gradiation

A new method of high-performance liquid chromatography (HPLC) analysis to quantify isomers of retinol, retinal and retinoic acid simultaneously was established. The HPLC system consisted of a silica gel absorption column and a linear gradient with two kinds of solvents containing n-Hexane, 2-propano...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-06, Vol.757 (2), p.365-368
Main Authors: Miyagi, Michiko, Yokoyama, Hirokazu, Shiraishi, Haruko, Matsumoto, Michinaga, Ishii, Hiromasa
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Isomerism
Medical sciences
Pharmacology. Drug treatments
Retinal
Retinaldehyde - analysis
Retinoic acid
Retinol
Spectrophotometry, Ultraviolet
Tretinoin - analysis
Vitamin A - analysis
Vitamins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new method of high-performance liquid chromatography (HPLC) analysis to quantify isomers of retinol, retinal and retinoic acid simultaneously was established. The HPLC system consisted of a silica gel absorption column and a linear gradient with two kinds of solvents containing n-Hexane, 2-propanol, and glacial acetic acid in different ratios. It separated six retinoic acid isomers (13- cis, 9- cis, all- trans, all- trans-4-oxo, 9- cis-4-oxo, 13- cis-4-oxo), three retinal isomers (13- cis-, 9- cis-, and all- trans) and two retinol isomers (13- cis- and all- trans). Human serum samples were subjected to this HPLC analysis and at least, all- trans retinol, 13- cis retinol, and all- trans retinoic acid were detectable. This HPLC system is useful for evaluating retinoic acid formation from retinol via a two-step oxidation pathway. Moreover, it could be applied to monitoring the concentrations of various retinoids, including all- trans retinoic acid in human sera.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(01)00158-X