Volatile Compounds from Escherichia coli O157:H7 and Their Absorption by Strawberry Fruit

• Published in: Journal of agricultural and food chemistry 2000-02, Vol.48 (2), p.413-417
• Main Authors: Yu, Keshun, Hamilton-Kemp, Thomas R, Archbold, Douglas D, Collins, Randall W, Newman, Melissa C
• Format: Article
• Language: English
• Subjects: Absorption; Biological and medical sciences; Chromatography, Gas; Escherichia coli O157 - metabolism; Food industries; Food microbiology; Fruit - metabolism; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf990576b

Volatile compounds emitted by cultures of two strains of the pathogenic bacterium Escherichia coli O157:H7 and a nonpathogenic strain of E. coli were trapped on Super-Q porous polymer and identified by GC−MS. The predominant compound produced by all three strains was indole with lesser amounts of other components including methyl ketones, 2-heptanone, 2-nonanone, 2-undecanone, and 2-tridecanone. The vapor-phase profiles of these strains were similar for most chemicals identified but differed with regard to ketones. Strawberry fruit was shown to be a suitable host for E. coli O157:H7 with the population of the bacterium either increasing or remaining stable after 3 days depending on inoculation level. Headspace analysis of the volatile compounds from inoculated fruit yielded no detectable quantity of indole. Strawberry fruit readily absorbed indole and other volatile compounds produced by the bacteria and in some cases metabolized the compounds to new volatile products. Thus, headspace “marker” compounds indicating possible bacterial contamination of fruit were largely removed from the vapor phase by the strawberries. Keywords: Indole; 2-heptanone; aroma; headspace; food safety; human pathogen