Influenza virus A stimulates expression of eotaxin by nasal epithelial cells

• Published in: Clinical and experimental allergy 2001-06, Vol.31 (6), p.873-880
• Main Authors: Kawaguchi, M., Kokubu, F., Kuga, H., Tomita, T., Matsukura, S., Suzaki, H., Huang, S.-K., Adachi, M.
• Format: Article
• Language: English
• Subjects: airway inflammation; Antibodies - pharmacology; Biological and medical sciences; CCR3 protein; Chemokine CCL11; Chemokines, CC; Cytokines - drug effects; Cytokines - genetics; Cytokines - immunology; Cytokines - pharmacology; Cytokines - radiation effects; eotaxin; Epithelial Cells - drug effects; Epithelial Cells - physiology; Gene Expression; Hemagglutinins, Viral - genetics; Hemagglutinins, Viral - radiation effects; Human viral diseases; Humans; Infectious diseases; Influenza A virus; Influenza A virus - genetics; influenza virus; Influenza, Human - genetics; Interferon-gamma - immunology; Interferon-gamma - pharmacology; interleukin 1^b; interleukin 1b; Interleukin-1 - immunology; Interleukin-1 - pharmacology; Medical sciences; nasal epithelial cells; Nasal Mucosa - cytology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - radiation effects; Tumor Necrosis Factor-alpha - immunology; Tumor Necrosis Factor-alpha - pharmacology; Ultraviolet Rays; Viral diseases; Viral diseases of the respiratory system and ent viral diseases
• ISSN: 0954-7894; 1365-2222
• DOI: 10.1046/j.1365-2222.2001.01103.x

Background Respiratory virus is one of the most common causes of airway inflammation, but its pathogenic mechanisms are not well understood. Eotaxin is a potent eosinophil chemoattractant and is a selective agonist for C‐C chemokine receptor 3 (CCR3). Although it has recently been demonstrated that epithelial cells express eotaxin, both in vivo and in vitro, there are few data concerning the expression in viral infection. Objects We hypothesized that eotaxin may play an important role in attracting inflammatory cells into the airway after viral infection and analysed whether viral infection induces eotaxin in nasal epithelial cells in vitro. Methods Nasal epithelial cells obtained from polypectomy for nasal polyp were infected with influenza virus A (subtype H3N2). The cells and supernatants were collected 8, 24 and 48 h after infection. Eotaxin mRNA was analysed by RT‐PCR. Eotaxin concentration in the supernatants was analysed by enzyme‐linked immunosorbent assay. We also examined a blocking assay to analyse the intervention of pro‐inflammatory cytokines, TNF‐α and IL‐1β in eotaxin production induced by influenza virus. Results The results showed that eotaxin was expressed constitutively in uninfected cells, but was up‐regulated for both mRNA and protein levels in infected cells. Blocking experiments using anti‐TNF‐α and anti‐IL‐1β antibodies showed no effects of these agents on the level of eotaxin. In addition, UV‐inactivated virus did not enhance the expression of eotaxin. Conclusions These results suggest that influenza virus A infection in nasal epithelial cells stimulates the expression of eotaxin, and may play an important role in the pathogenesis of airway inflammation by inducing eotaxin.