Genomic organization and characterization of the promoter region of murine GSTM2 gene

In this study, we have isolated the genomic clone of the murine GSTM2 gene and determined its sequence. Consistent with the class mu genefamily, the mGSTM2 gene consists of eight exons. The exon-intron boundaries and the distribution of coding sequences within the exons of the known GST class mu fam...

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Bibliographic Details
Published in:Gene 2001-05, Vol.270 (1), p.221-229
Main Authors: Kumar, Atul, Reddy, E.Premkumar
Format: Article
Language:English
Subjects:
Animals
Base Sequence
DNA - chemistry
DNA - genetics
DNA - isolation & purification
Exons
Genes - genetics
Genomic structure
Glutathione S-transferase mu
Glutathione Transferase - genetics
GSTM2 gene
Introns
Luciferases - genetics
Luciferases - metabolism
Mice
Mice, Inbred Strains
Molecular Sequence Data
Mutation
Myb responsive element
Promoter Regions, Genetic - genetics
Proto-Oncogene Proteins c-myb - genetics
Proto-Oncogene Proteins c-myb - physiology
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Deletion
Sequence Homology, Nucleic Acid
t-Myb protein
Transcriptional Activation
Tumor Cells, Cultured
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Summary:In this study, we have isolated the genomic clone of the murine GSTM2 gene and determined its sequence. Consistent with the class mu genefamily, the mGSTM2 gene consists of eight exons. The exon-intron boundaries and the distribution of coding sequences within the exons of the known GST class mu family members were found to follow a similar pattern suggesting that various members of this family have originated from a single primordial gene by duplication and the structure has been closely maintained through evolution. By primer extension, the start of transcription was determined to be 40 bp upstream of the initial AUG codon. To gain an understanding of the mGSTM2 regulation, we have also cloned and analyzed its promoter region. Maximal activity was observed in a 170 bp 5′-flanking region. The activity was decreased by 3-fold in a 402 bp 5′-flanking region suggesting the presence of repressor elements. While no TATA box was identified, the presence of an SP1 site at position −38 was noted. Deletion of this SP1 site completely abrogated promoter activity. The promoter contained eight putative Myb responsive elements and its transcriptional activity was upregulated by t-Myb but not by c-Myb.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(01)00473-5