cDNA cloning and genetic polymorphism of the swine major histocompatibility complex (SLA) class II DMA gene

• Published in: Animal genetics 2001-04, Vol.32 (2), p.73-77
• Main Authors: Ando, A., Kawata, H., Murakami, T., Shigenari, A., Shiina, T., Sada, M., Tsuji, T., Toriu, A., Nakanishi, Y., Mitsuhashi, T., Sekikawa, K., Inoko, H.
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; Animals; antigen presentation; Base Sequence; Cloning, Molecular; DM α chain; DNA, Complementary - genetics; Exons - genetics; Genes, MHC Class II - genetics; Genotype; Histocompatibility Antigens Class II - chemistry; Histocompatibility Antigens Class II - genetics; miniature pig; Molecular Sequence Data; polymorphism; Polymorphism, Genetic - genetics; Sequence Homology; SLA; SLA-DMA gene; Swine - genetics; Swine - immunology
• ISSN: 0268-9146; 1365-2052
• DOI: 10.1046/j.1365-2052.2001.00733.x

cDNA clones corresponding to the swine histocompatibility complex (SLA: swine leucocyte antigen)‐DM α chain were isolated using the polymerase chain reaction (PCR) products from the third exon in the human HLA‐DMA gene as a probe. Amino acid comparative analysis revealed that these clones were more closely related to the bovine and human DMA genes than to the other swine class II genes α chain genes, DRA, DQA and DOA. These results suggest that the SLA‐DMA gene is expressed and may function, like HLA‐DM, as an important modulator in class II restricted antigen processing in swine. Furthermore, based on the sequences and PCR–restriction fragment length polymorphism (PCR–RFLP) patterns in the SLA‐DMA gene, no allelic variation was recognized in the second exon, but five allelic variations were recognized in the third exon in five different breeds of swine. These DMA alleles were defined by variation at four nucleotide positions. Two of these alleles resulted in an amino acid substitution. These results suggest that SLA‐DMA has little polymorphism as observed in HLA‐DMA and mouse H2‐Ma.