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THE HAMSTER HEART IS RESISTANT TO CALCIUM PARADOX
Reintroduction of Ca2+or modification of internal Ca2+stores by caffeine results in widespread irreversible injury. The adult golden hamster, however, is immune to such insult and the present report investigates the phenomenon. Isolated Langendorff perfused hamster and rat heart were subjected to 15...
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| Published in: | Pharmacological research 2000-04, Vol.41 (4), p.475-481, Article 475 |
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| container_end_page | 481 |
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| container_start_page | 475 |
| container_title | Pharmacological research |
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| creator | RAY, MADHUR SRIVASTAVA, SHEKHAR MAITRA, SHYAMAL CHANDRA DUBEY, MANGAL PRASAD |
| description | Reintroduction of Ca2+or modification of internal Ca2+stores by caffeine results in widespread irreversible injury. The adult golden hamster, however, is immune to such insult and the present report investigates the phenomenon. Isolated Langendorff perfused hamster and rat heart were subjected to 15 min Ca2+-free perfusion followed by 30 min of Ca2+perfusion at 37°C. Caffeine was introduced during Ca2+-free perfusion in a number of experiments. Papillary muscles were processed for the ultra-structural study. The hamster heart did not exhibit the calcium paradox state whereas rat heart did. Hamster heart treated with caffeine either throughout or 5 min after starting Ca2+-free perfusion showed 70%±8 or 65%±8.42 recovery, respectively, when Ca2+reperfusion was performed. Ultrastructure of muscle from both groups showed relaxed myocytes with slight disorientation of the sarcomere register. This disorientation was not seen in hamster hearts undergoing the conventional calcium paradox protocol. The hamster cardiac muscle is remarkably tolerant to [Ca2+]i loading either induced by Ca2+reperfusion or caffeine-induced sarcoplasmic reticulum Ca2+release. Structural and functional characterization of Ca2+depletion and repletion in the hamster heart have been discussed. |
| doi_str_mv | 10.1006/phrs.1999.0607 |
| format | article |
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The adult golden hamster, however, is immune to such insult and the present report investigates the phenomenon. Isolated Langendorff perfused hamster and rat heart were subjected to 15 min Ca2+-free perfusion followed by 30 min of Ca2+perfusion at 37°C. Caffeine was introduced during Ca2+-free perfusion in a number of experiments. Papillary muscles were processed for the ultra-structural study. The hamster heart did not exhibit the calcium paradox state whereas rat heart did. Hamster heart treated with caffeine either throughout or 5 min after starting Ca2+-free perfusion showed 70%±8 or 65%±8.42 recovery, respectively, when Ca2+reperfusion was performed. Ultrastructure of muscle from both groups showed relaxed myocytes with slight disorientation of the sarcomere register. This disorientation was not seen in hamster hearts undergoing the conventional calcium paradox protocol. The hamster cardiac muscle is remarkably tolerant to [Ca2+]i loading either induced by Ca2+reperfusion or caffeine-induced sarcoplasmic reticulum Ca2+release. Structural and functional characterization of Ca2+depletion and repletion in the hamster heart have been discussed.</description><identifier>ISSN: 1043-6618</identifier><identifier>EISSN: 1096-1186</identifier><identifier>DOI: 10.1006/phrs.1999.0607</identifier><identifier>PMID: 10704273</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Caffeine - pharmacology ; Calcium - deficiency ; Calcium - metabolism ; Cricetinae ; electron microscopy, gap junctions, myocytes, hamster heart, calcium paradox ; Heart - drug effects ; In Vitro Techniques ; Male ; Mesocricetus ; Microscopy, Electron ; Myocardial Contraction - drug effects ; Myocardial Reperfusion Injury - metabolism ; Myocardial Reperfusion Injury - pathology ; Myocardium - cytology ; Myocardium - metabolism ; Myocardium - pathology ; Papillary Muscles - drug effects ; Phosphodiesterase Inhibitors - pharmacology ; Rats ; Rats, Sprague-Dawley ; Sarcoplasmic Reticulum - drug effects ; Sarcoplasmic Reticulum - metabolism ; Tissue Fixation</subject><ispartof>Pharmacological research, 2000-04, Vol.41 (4), p.475-481, Article 475</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-c03e848173749eb9bed6e70a5cea830009308f002e89487bec123153cfe71dd43</citedby><cites>FETCH-LOGICAL-c340t-c03e848173749eb9bed6e70a5cea830009308f002e89487bec123153cfe71dd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1043661899906072$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3547,27923,27924,45779</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10704273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RAY, MADHUR</creatorcontrib><creatorcontrib>SRIVASTAVA, SHEKHAR</creatorcontrib><creatorcontrib>MAITRA, SHYAMAL CHANDRA</creatorcontrib><creatorcontrib>DUBEY, MANGAL PRASAD</creatorcontrib><title>THE HAMSTER HEART IS RESISTANT TO CALCIUM PARADOX</title><title>Pharmacological research</title><addtitle>Pharmacol Res</addtitle><description>Reintroduction of Ca2+or modification of internal Ca2+stores by caffeine results in widespread irreversible injury. The adult golden hamster, however, is immune to such insult and the present report investigates the phenomenon. Isolated Langendorff perfused hamster and rat heart were subjected to 15 min Ca2+-free perfusion followed by 30 min of Ca2+perfusion at 37°C. Caffeine was introduced during Ca2+-free perfusion in a number of experiments. Papillary muscles were processed for the ultra-structural study. The hamster heart did not exhibit the calcium paradox state whereas rat heart did. Hamster heart treated with caffeine either throughout or 5 min after starting Ca2+-free perfusion showed 70%±8 or 65%±8.42 recovery, respectively, when Ca2+reperfusion was performed. Ultrastructure of muscle from both groups showed relaxed myocytes with slight disorientation of the sarcomere register. This disorientation was not seen in hamster hearts undergoing the conventional calcium paradox protocol. The hamster cardiac muscle is remarkably tolerant to [Ca2+]i loading either induced by Ca2+reperfusion or caffeine-induced sarcoplasmic reticulum Ca2+release. Structural and functional characterization of Ca2+depletion and repletion in the hamster heart have been discussed.</description><subject>Animals</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - deficiency</subject><subject>Calcium - metabolism</subject><subject>Cricetinae</subject><subject>electron microscopy, gap junctions, myocytes, hamster heart, calcium paradox</subject><subject>Heart - drug effects</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mesocricetus</subject><subject>Microscopy, Electron</subject><subject>Myocardial Contraction - drug effects</subject><subject>Myocardial Reperfusion Injury - metabolism</subject><subject>Myocardial Reperfusion Injury - pathology</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - pathology</subject><subject>Papillary Muscles - drug effects</subject><subject>Phosphodiesterase Inhibitors - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sarcoplasmic Reticulum - drug effects</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Tissue Fixation</subject><issn>1043-6618</issn><issn>1096-1186</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kMFPwjAUhxujEUSvHs1O3oavtKztccHpSEQMG4m3ZnSPWAMM22Hif--WcTAmnPoO3-9L8xFyS2FIAaKH_YfzQ6qUGkIE4oz0KagopFRG5-3NWRhFVPbIlfefAKA4hUvSoyCAjwTrE5qnSZDGsyxPFkGaxIs8mGbBIsmmWR6_5kE-Dybxy2S6nAVv8SJ-nL9fk4t1sfF4c3wHZPmU5JM0fJk_Txs2NIxDHRpgKLmkggmucKVWWEYooBgbLCRrv8JArgFGKBWXYoWGjhgdM7NGQcuSswG577x7V30d0Nd6a73BzabYYXXwWoAaj0HQBhx2oHGV9w7Xeu_stnA_moJuI-k2km4j6TZSM7g7mg-rLZZ_8K5KA_B_RmProrbVrnaF3Zz2ym6GTZZvi057Y3FnsLQOTa3Lyp6a_gKuM4Fw</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>RAY, MADHUR</creator><creator>SRIVASTAVA, SHEKHAR</creator><creator>MAITRA, SHYAMAL CHANDRA</creator><creator>DUBEY, MANGAL PRASAD</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000401</creationdate><title>THE HAMSTER HEART IS RESISTANT TO CALCIUM PARADOX</title><author>RAY, MADHUR ; SRIVASTAVA, SHEKHAR ; MAITRA, SHYAMAL CHANDRA ; DUBEY, MANGAL PRASAD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-c03e848173749eb9bed6e70a5cea830009308f002e89487bec123153cfe71dd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - deficiency</topic><topic>Calcium - metabolism</topic><topic>Cricetinae</topic><topic>electron microscopy, gap junctions, myocytes, hamster heart, calcium paradox</topic><topic>Heart - drug effects</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mesocricetus</topic><topic>Microscopy, Electron</topic><topic>Myocardial Contraction - drug effects</topic><topic>Myocardial Reperfusion Injury - metabolism</topic><topic>Myocardial Reperfusion Injury - pathology</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - pathology</topic><topic>Papillary Muscles - drug effects</topic><topic>Phosphodiesterase Inhibitors - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sarcoplasmic Reticulum - drug effects</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Tissue Fixation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAY, MADHUR</creatorcontrib><creatorcontrib>SRIVASTAVA, SHEKHAR</creatorcontrib><creatorcontrib>MAITRA, SHYAMAL CHANDRA</creatorcontrib><creatorcontrib>DUBEY, MANGAL PRASAD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmacological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RAY, MADHUR</au><au>SRIVASTAVA, SHEKHAR</au><au>MAITRA, SHYAMAL CHANDRA</au><au>DUBEY, MANGAL PRASAD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE HAMSTER HEART IS RESISTANT TO CALCIUM PARADOX</atitle><jtitle>Pharmacological research</jtitle><addtitle>Pharmacol Res</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>41</volume><issue>4</issue><spage>475</spage><epage>481</epage><pages>475-481</pages><artnum>475</artnum><issn>1043-6618</issn><eissn>1096-1186</eissn><abstract>Reintroduction of Ca2+or modification of internal Ca2+stores by caffeine results in widespread irreversible injury. The adult golden hamster, however, is immune to such insult and the present report investigates the phenomenon. Isolated Langendorff perfused hamster and rat heart were subjected to 15 min Ca2+-free perfusion followed by 30 min of Ca2+perfusion at 37°C. Caffeine was introduced during Ca2+-free perfusion in a number of experiments. Papillary muscles were processed for the ultra-structural study. The hamster heart did not exhibit the calcium paradox state whereas rat heart did. Hamster heart treated with caffeine either throughout or 5 min after starting Ca2+-free perfusion showed 70%±8 or 65%±8.42 recovery, respectively, when Ca2+reperfusion was performed. Ultrastructure of muscle from both groups showed relaxed myocytes with slight disorientation of the sarcomere register. This disorientation was not seen in hamster hearts undergoing the conventional calcium paradox protocol. The hamster cardiac muscle is remarkably tolerant to [Ca2+]i loading either induced by Ca2+reperfusion or caffeine-induced sarcoplasmic reticulum Ca2+release. Structural and functional characterization of Ca2+depletion and repletion in the hamster heart have been discussed.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>10704273</pmid><doi>10.1006/phrs.1999.0607</doi><tpages>7</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection; ScienceDirect Journals |
| subjects | Animals Caffeine - pharmacology Calcium - deficiency Calcium - metabolism Cricetinae electron microscopy, gap junctions, myocytes, hamster heart, calcium paradox Heart - drug effects In Vitro Techniques Male Mesocricetus Microscopy, Electron Myocardial Contraction - drug effects Myocardial Reperfusion Injury - metabolism Myocardial Reperfusion Injury - pathology Myocardium - cytology Myocardium - metabolism Myocardium - pathology Papillary Muscles - drug effects Phosphodiesterase Inhibitors - pharmacology Rats Rats, Sprague-Dawley Sarcoplasmic Reticulum - drug effects Sarcoplasmic Reticulum - metabolism Tissue Fixation |
| title | THE HAMSTER HEART IS RESISTANT TO CALCIUM PARADOX |
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