Comparative multi-color flow cytometric analysis of cell surface antigens in bone marrow hematopoietic progenitors between refractory anemia and aplastic anemia

Refractory anemia (RA) in myelodysplastic syndrome (MDS) without prominent dysplasia closely resemble the mild type of aplastic anemia (AA) in their hematological features. This sometimes makes it difficult to distinguish clearly between the two diseases. Using the multi-color flow cytometric techni...

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Bibliographic Details
Published in:Leukemia research 2000-04, Vol.24 (4), p.359-366
Main Authors: Otawa, Masahiko, Kawanishi, Yoshikazu, Iwase, Osamu, Shoji, Nahoko, Miyazawa, Keisuke, Ohyashiki, Kazuma
Format: Article
Language:English
Subjects:
ADP-ribosyl Cyclase
ADP-ribosyl Cyclase 1
Adult
Aged
Anemia, Aplastic - immunology
Anemia, Refractory - immunology
Antigens, CD
Antigens, CD34 - analysis
Antigens, Differentiation - analysis
Antigens, Surface - analysis
Aplastic anemia
Biological and medical sciences
Bone marrow
Bone Marrow Cells - immunology
CD34
Flow Cytometry
Hematology
Hematopoietic progenitors
Hematopoietic Stem Cells - immunology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Leukocyte Common Antigens - analysis
Medical sciences
Membrane Glycoproteins
Middle Aged
Myelodysplastic syndrome
NAD+ Nucleosidase - analysis
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Refractory anemia
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Summary:Refractory anemia (RA) in myelodysplastic syndrome (MDS) without prominent dysplasia closely resemble the mild type of aplastic anemia (AA) in their hematological features. This sometimes makes it difficult to distinguish clearly between the two diseases. Using the multi-color flow cytometric technique, we compared cell surface antigen expression patterns on bone marrow hematopoietic progenitor cells which were isolated as a CD34 positive- CD45 dull positive with low side scatter intensity (CD34 +CD45 dull+SSC low) population in flow cytogram between RA ( n=12) and AA ( n=11). The antigens analyzed in CD34 +CD45 dull+SSC low mononuclear cells were: CD38 and CD71 for cell growth-related antigens, CD 33 and CD13 for myeloid and monocytoid lineage-associated antigens, CD7 and CD19 for lymphoid lineage, and CD14 for a monocytic lineage specific antigen. The percentages of CD34 +CD45 dull+SSC low cells in bone marrow non-erythroid mononuclear cells, and the expression frequencies of CD38, CD71, CD33 and CD13 antigens in CD34 +CD45 dull+SSC low progenitors were all significantly decreased in AA compared to normal bone marrows ( n=7) ( P
ISSN:0145-2126
1873-5835
DOI:10.1016/S0145-2126(99)00194-0