Genetic transformation of the housefly Musca domestica with the lepidopteran derived transposon piggyBac

The piggyBac transposable element was successfully used for stable genetic transformation of the housefly Musca domestica. The construct contains the EGFP marker under the control of Pax‐6 binding sites, which can drive eye‐specific expression in insect species as distantly related as Drosophila mel...

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Bibliographic Details
Published in:Insect molecular biology 2001-04, Vol.10 (2), p.113-119
Main Authors: Hediger, M, Niessen, M, Wimmer, E. A, Dübendorfer, A, Bopp, D
Format: Article
Language:English
Subjects:
Animals
Baculoviridae
binding sites
DNA Transposable Elements
Drosophila melanogaster
Gene Expression
gene transfer
Genetic Markers
genetic transformation
Genetic Vectors
Green Fluorescent Proteins
Houseflies - genetics
insects
Larva
Lepidoptera
Luminescent Proteins - genetics
Moths - genetics
Musca domestica
piggyBac
Tenebrionidae
Transformation, Genetic
transgenesis
transposition (genetics)
transposons
Tribolium castaneum
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Summary:The piggyBac transposable element was successfully used for stable genetic transformation of the housefly Musca domestica. The construct contains the EGFP marker under the control of Pax‐6 binding sites, which can drive eye‐specific expression in insect species as distantly related as Drosophila melanogaster and Tribolium castaneum[Berghammer, A.J., Klingler, M. and Wimmer, E.A. (1999)Nature 402: 370–371]. We obtained seven independent integration events among 41 fertile G0Musca flies. Most of the transformed lines contained two or more chromosomal insertions of the EGFP marker which were stably inherited over more than 15 generations. piggyBac‐mediated transposition was verified by identifying the characteristic TTAA duplication at the insertion sites. This first report of stable transmission of a genetic marker in Musca confirms the use of this vector‐marker system for effective gene transfer in a broad range of insect species.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.2001.00243.x