Medium-chain triglycerides inhibit free radical formation and TNF-alpha production in rats given enteral ethanol

This study determined whether free radical formation by the liver, tumor necrosis factor (TNF)-alpha production by isolated Kupffer cells, and plasma endotoxin are affected by dietary saturated fat. Rats were fed enteral ethanol and corn oil (E-CO) or medium-chain triglycerides (E-MCT) and control r...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology: Gastrointestinal and liver physiology 2000-03, Vol.278 (3), p.G467-G476
Main Authors: Kono, H, Enomoto, N, Connor, H D, Wheeler, M D, Bradford, B U, Rivera, C A, Kadiiska, M B, Mason, R P, Thurman, R G
Format: Article
Language:English
Subjects:
Animals
Aspartate Aminotransferases - blood
Body Weight - drug effects
Corn Oil - pharmacology
Dietary Fats, Unsaturated - pharmacology
Endotoxins - blood
Ethanol - administration & dosage
Ethanol - metabolism
Ethanol - pharmacology
Ethanol - urine
Free Radicals - antagonists & inhibitors
Intestine, Small - physiology
Intestines - metabolism
Intestines - pathology
Kupffer Cells - metabolism
Lipopolysaccharide Receptors - metabolism
Liver - drug effects
Liver - pathology
Male
Osmolar Concentration
Permeability
Rats
Rats, Wistar
Triglycerides - chemistry
Triglycerides - pharmacology
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study determined whether free radical formation by the liver, tumor necrosis factor (TNF)-alpha production by isolated Kupffer cells, and plasma endotoxin are affected by dietary saturated fat. Rats were fed enteral ethanol and corn oil (E-CO) or medium-chain triglycerides (E-MCT) and control rats received corn oil (C-CO) or medium-chain triglycerides (C-MCT) for 2 wk. E-CO rats developed moderate fatty infiltration and slight inflammation; however, E-MCT prevented liver injury. Serum aspartate aminotransferase levels, gut permeability, and plasma endotoxin doubled with E-CO but were blunted approximately 50% with E-MCT. In Kupffer cells from E-CO rats, intracellular calcium was elevated by lipopolysaccharide (LPS) in a dose-dependent manner. In cells from E-MCT rats, increases were blunted by approximately 40-50% at all concentrations of LPS. The LPS-induced increase in TNF-alpha production by Kupffer cells was dose dependent and was blunted by 40% by MCT. E-CO increased radical adducts and was reduced approximately 50% by MCT. MCT prevent early alcohol-induced liver injury, in part, by inhibition of free radical formation and TNF-alpha production by inhibition of endotoxin-mediated activation of Kupffer cells.
ISSN:0193-1857
1522-1547
DOI:10.1152/ajpgi.2000.278.3.g467