Serum withdrawal-induced apoptosis in thyroid cells is caused by loss of fibronectin-integrin interaction

In some cell types, including a fetal thyroid cell line, denial of adhesion to extracellular matrix induces a type of apoptosis called anoikis. Serum withdrawal in dog and transformed rat thyroid cells also induces programmed cell death. Because serum can stimulate cells to produce some components o...

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Bibliographic Details
Published in:The journal of clinical endocrinology and metabolism 2000-03, Vol.85 (3), p.1188-1193
Main Authors: DI MATOLA, T, MUELLER, F, FENZI, G, ROSSI, G, BIFULCO, M, MARZANO, L. A, VITALE, M
Format: Article
Language:English
Subjects:
Ageing, cell death
Annexin A5 - metabolism
Apoptosis - physiology
Biological and medical sciences
Cell Adhesion
Cell physiology
Cell Survival - physiology
Cells, Cultured
Culture Media, Serum-Free
DNA - biosynthesis
DNA - genetics
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix - physiology
Fibronectins - biosynthesis
Fibronectins - physiology
Flow Cytometry
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Integrins - physiology
Molecular and cellular biology
Space life sciences
Thyroid Gland - cytology
Thyroid Gland - physiology
Thyrotropin - pharmacology
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Summary:In some cell types, including a fetal thyroid cell line, denial of adhesion to extracellular matrix induces a type of apoptosis called anoikis. Serum withdrawal in dog and transformed rat thyroid cells also induces programmed cell death. Because serum can stimulate cells to produce some components of the extracellular matrix, it was of interest to determine the role of the matrix in the apoptosis induced by serum withdrawal in normal human thyroid cells in primary culture. The present report demonstrates that thyroid cells selectively produce and deposit insoluble fibronectin (FN) only when stimulated by serum. Adhesion in the presence of serum is dependent upon integrin-FN interaction. Serum withdrawal determines a degradation of the insoluble FN deposited and a detachment of the cells from the plates. In these conditions, cells undergo anoikis, demonstrated by DNA fragmentation and annexin V staining. Apoptosis was prevented by exogenous FN immobilized onto the plates. These results indicate that serum withdrawal induces apoptosis in human thyroid cells, determining FN degradation and loss of cell-matrix adhesion.
ISSN:0021-972X
DOI:10.1210/jc.85.3.1188