Separation of Nontarget Compounds by DNA Aptamers

The ability of DNA aptamers to separate nontarget compounds is demonstrated. Two G-quartet forming aptamers, a 15-mer and a 20-mer, were covalently linked to fused silica capillary columns to serve as stationary-phase reagents in capillary electrochromatography. Separations of binary mixtures of ami...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2000-02, Vol.72 (4), p.827-831
Main Authors: Kotia, Ruchi Bhatnagar, Li, Lijuan, McGown, Linda B
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biochemistry
Biological and medical sciences
Deoxyribonucleic acid
DNA
DNA - chemistry
Electrophoresis, Capillary - methods
Fundamental and applied biological sciences. Psychology
Nucleic Acid Conformation
Oligonucleotides - chemistry
Polycyclic Aromatic Hydrocarbons - isolation & purification
Stereoisomerism
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Summary:The ability of DNA aptamers to separate nontarget compounds is demonstrated. Two G-quartet forming aptamers, a 15-mer and a 20-mer, were covalently linked to fused silica capillary columns to serve as stationary-phase reagents in capillary electrochromatography. Separations of binary mixtures of amino acids (D-trp and D-tyr), enantiomers (D-trp and L-trp), and polycyclic aromatic hydrocarbons were achieved. Aptamers offer several attractive features for stationary-phase reagents, including ease of synthesis and of attachment to surfaces and modification of their binding properties through minor changes in sequence.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac991112f