Vasopressin regulates endothelin-B receptor in rat inner medullary collecting duct

Previous studies have shown that endothelin (ET) antagonizes the actions of arginine vasopressin (AVP) in the renal collecting ducts. On the other hand, the effects of AVP on ET function within the collecting ducts of the kidney have not been investigated extensively. Using isolated inner medullary...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 2000-03, Vol.278 (3), p.F369-F374
Main Authors: Wong, N L, Wong, B P, Tsui, J K
Format: Article
Language:English
Subjects:
Animals
Arginine Vasopressin - pharmacology
Colforsin - pharmacology
Cyclic AMP - analogs & derivatives
Cyclic AMP - pharmacology
Cyclic GMP - metabolism
Endothelin-1 - metabolism
Kidney Medulla
Kidney Tubules, Collecting - cytology
Kidney Tubules, Collecting - metabolism
Male
Rats
Rats, Wistar
Receptor, Endothelin B
Receptors, Endothelin - genetics
Receptors, Endothelin - metabolism
RNA, Messenger - metabolism
Thionucleotides - pharmacology
Time Factors
Vasopressins - physiology
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Summary:Previous studies have shown that endothelin (ET) antagonizes the actions of arginine vasopressin (AVP) in the renal collecting ducts. On the other hand, the effects of AVP on ET function within the collecting ducts of the kidney have not been investigated extensively. Using isolated inner medullary collecting ducts (IMCD), we examined the possibility that a decrease in ET(B) receptor mRNA accompanied AVP-induced downregulation of ET(B) receptors. Binding studies revealed that overnight incubation of rat IMCD cells with AVP significantly reduced the maximal binding capacity (B(max)) of ET. Activation of adenylate cyclase by forskolin decreased the total ET(B) receptor density by approximately 42% but did not affect the density of ET(A) receptors. The Rp diastereoisomer of adenosine 3', 5'-cyclic monophosphothionate, Rp-cAMPS (a specific inhibitor of protein kinase A), blocked the AVP-induced reduction in ET receptor density. Using competitive PCR method, we also observed downregulation of ET(B) receptor mRNA in IMCD treated with AVP. Additional studies were done with IMCD to determine whether AVP inhibited the ET-induced accumulation of cGMP. We saw a reduction in ET-induced cGMP accumulation when IMCD was incubated overnight with AVP. This inhibition of ET-induced accumulation of cGMP was blocked by Rp-cAMPS. These results suggest that AVP regulates ET(B) receptor expression in IMCD.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.2000.278.3.F369