Chip Interacts with Diverse Homeodomain Proteins and Potentiates Bicoid Activity in vivo

The Drosophila protein Chip potentiates activation by several enhancers and is required for embryonic segmentation. Chip and its mammalian homologs interact with and promote dimerization of nuclear LIM proteins. No known Drosophila LIM proteins, however, are required for segmentation, nor for expres...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-03, Vol.97 (6), p.2686-2691
Main Authors: Torigoi, E, Bennani-Baiti, I M, Rosen, C, Gonzalez, K, Morcillo, P, Ptashne, M, Dorsett, D
Format: Article
Language:English
Subjects:
Animals
Bicoid protein
Binding sites
Biological Sciences
Chip protein
Chromatography, Affinity
Complementary DNA
DNA-Binding Proteins - metabolism
Drosophila
Drosophila - embryology
Drosophila - metabolism
Drosophila melanogaster
Drosophila Proteins
Embryos
Enhancer Elements, Genetic
Genes
Homeodomain Proteins - chemistry
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Insect Proteins - chemistry
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
LIM protein
Models, Biological
Molecular interactions
Molecules
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Polymerase chain reaction
Promoter Regions, Genetic
Protein Binding
Protein Structure, Tertiary
Proteins
Recombinant Fusion Proteins - metabolism
Repressor Proteins
Trans-Activators - chemistry
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription, Genetic
Transcriptional Activation
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Drosophila protein Chip potentiates activation by several enhancers and is required for embryonic segmentation. Chip and its mammalian homologs interact with and promote dimerization of nuclear LIM proteins. No known Drosophila LIM proteins, however, are required for segmentation, nor for expression of most genes known to be regulated by Chip. Here we show that Chip also interacts with diverse homeodomain proteins using residues distinct from those that interact with LIM proteins, and that Chip potentiates activity of one of these homeodomain proteins in Drosophila embryos and in yeast. These and other observations help explain the roles of Chip in segmentation and suggest a model to explain how Chip potentiates activation by diverse enhancers.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.050586397