Mice Lacking Dad1, the Defender against Apoptotic Death-1, Express Abnormal N-Linked Glycoproteins and Undergo Increased Embryonic Apoptosis

Dad1 has been shown to play a role in preventing apoptotic cell death and in regulating levels of N-linked glycosylation in Saccharomyces cerevisiae and the BHK hamster cell line. To address the in vivo role of Dad1 in these processes during multicellular development, we have analyzed mice carrying...

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Bibliographic Details
Published in:Developmental biology 2000-04, Vol.220 (1), p.76-84
Main Authors: Hong, N.A., Flannery, M., Hsieh, S.N., Cado, D., Pedersen, R., Winoto, A.
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - genetics
Apoptosis - physiology
Apoptosis Regulatory Proteins
Caenorhabditis elegans Proteins
Cricetinae
Dad1
Embryonic and Fetal Development - genetics
Embryonic and Fetal Development - physiology
Female
Gene Expression Regulation, Developmental
Glycoproteins - genetics
Glycoproteins - physiology
Glycosylation
knockout
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Phenotype
Pregnancy
Reichert's membrane
Repressor Proteins - genetics
Repressor Proteins - physiology
visceral endoderm
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Summary:Dad1 has been shown to play a role in preventing apoptotic cell death and in regulating levels of N-linked glycosylation in Saccharomyces cerevisiae and the BHK hamster cell line. To address the in vivo role of Dad1 in these processes during multicellular development, we have analyzed mice carrying a null allele for Dad1. Embryos homozygous for this mutation express abnormal N-glycosylated proteins and are developmentally delayed by embryonic day 7.5. Such mutants exhibit aberrant morphology, impaired mesodermal development, and increased levels of apoptosis in specific tissues. These defects culminate in homozygous embryos failing to turn the posterior axis and subsequent lethality by embryonic day 10.5. Thus, Dad1 is required for proper processing of N-linked glycoproteins and for certain cell survival in the mouse.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.2000.9615