Purification and characterization of UDP-glucuronate: baicalein 7- O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures

• Published in: Phytochemistry (Oxford) 2000-03, Vol.53 (5), p.533-538
• Main Authors: Nagashima, Shigeyuki, Hirotani, Masao, Yoshikawa, Takafumi
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Baicalein; Baicalin; Biological and medical sciences; Cell culture; Chromatography, Gel; Drugs, Chinese Herbal - chemistry; Electrophoresis, Polyacrylamide Gel; Enzymes; Enzymes and enzyme inhibitors; Flavanones; Flavonoids - chemistry; Fundamental and applied biological sciences. Psychology; Glucuronosyltransferase; Glucuronosyltransferase - chemistry; Glucuronosyltransferase - isolation & purification; Lamiaceae; Lamiaceae - chemistry; Metabolism; Plant physiology and development; Plant Proteins - chemistry; Plant Proteins - isolation & purification; Plant Roots - chemistry; Scutellaria baicalensis; Substrate Specificity; Transferases; Uridine Diphosphate Glucuronic Acid - chemistry
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(99)00593-2

UDP-glucuronate: baicalein 7- O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and chromatofocusing procedures to apparent homogeneity. The M r was estimated to be 110 kDa by gel filtration chromatography with a 52 kDa subunit by SDS-PAGE. The isoelectric point was pH 4.8. UBGAT was specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).