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Purification and characterization of UDP-glucuronate: baicalein 7- O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures
UDP-glucuronate: baicalein 7- O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and...
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| Published in: | Phytochemistry (Oxford) 2000-03, Vol.53 (5), p.533-538 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c442t-e90659f72d4c030e2e9a391a9926e351c0ae616d9a217a5c0ac6398c0196ac5d3 |
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| cites | cdi_FETCH-LOGICAL-c442t-e90659f72d4c030e2e9a391a9926e351c0ae616d9a217a5c0ac6398c0196ac5d3 |
| container_end_page | 538 |
| container_issue | 5 |
| container_start_page | 533 |
| container_title | Phytochemistry (Oxford) |
| container_volume | 53 |
| creator | Nagashima, Shigeyuki Hirotani, Masao Yoshikawa, Takafumi |
| description | UDP-glucuronate: baicalein 7-
O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of
Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and chromatofocusing procedures to apparent homogeneity. The
M
r
was estimated to be 110 kDa by gel filtration chromatography with a 52 kDa subunit by SDS-PAGE. The isoelectric point was pH 4.8. UBGAT was specific to UDP-glucuronic acid as a sugar donor and flavones with substitution
ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe). |
| doi_str_mv | 10.1016/S0031-9422(99)00593-2 |
| format | article |
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O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of
Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and chromatofocusing procedures to apparent homogeneity. The
M
r
was estimated to be 110 kDa by gel filtration chromatography with a 52 kDa subunit by SDS-PAGE. The isoelectric point was pH 4.8. UBGAT was specific to UDP-glucuronic acid as a sugar donor and flavones with substitution
ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/S0031-9422(99)00593-2</identifier><identifier>PMID: 10724177</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; Baicalein ; Baicalin ; Biological and medical sciences ; Cell culture ; Chromatography, Gel ; Drugs, Chinese Herbal - chemistry ; Electrophoresis, Polyacrylamide Gel ; Enzymes ; Enzymes and enzyme inhibitors ; Flavanones ; Flavonoids - chemistry ; Fundamental and applied biological sciences. Psychology ; Glucuronosyltransferase ; Glucuronosyltransferase - chemistry ; Glucuronosyltransferase - isolation & purification ; Lamiaceae ; Lamiaceae - chemistry ; Metabolism ; Plant physiology and development ; Plant Proteins - chemistry ; Plant Proteins - isolation & purification ; Plant Roots - chemistry ; Scutellaria baicalensis ; Substrate Specificity ; Transferases ; Uridine Diphosphate Glucuronic Acid - chemistry</subject><ispartof>Phytochemistry (Oxford), 2000-03, Vol.53 (5), p.533-538</ispartof><rights>2000 Elsevier Science Ltd</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-e90659f72d4c030e2e9a391a9926e351c0ae616d9a217a5c0ac6398c0196ac5d3</citedby><cites>FETCH-LOGICAL-c442t-e90659f72d4c030e2e9a391a9926e351c0ae616d9a217a5c0ac6398c0196ac5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1284789$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10724177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagashima, Shigeyuki</creatorcontrib><creatorcontrib>Hirotani, Masao</creatorcontrib><creatorcontrib>Yoshikawa, Takafumi</creatorcontrib><title>Purification and characterization of UDP-glucuronate: baicalein 7- O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>UDP-glucuronate: baicalein 7-
O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of
Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and chromatofocusing procedures to apparent homogeneity. The
M
r
was estimated to be 110 kDa by gel filtration chromatography with a 52 kDa subunit by SDS-PAGE. The isoelectric point was pH 4.8. UBGAT was specific to UDP-glucuronic acid as a sugar donor and flavones with substitution
ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Baicalein</subject><subject>Baicalin</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Chromatography, Gel</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzymes</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Flavanones</subject><subject>Flavonoids - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucuronosyltransferase</subject><subject>Glucuronosyltransferase - chemistry</subject><subject>Glucuronosyltransferase - isolation & purification</subject><subject>Lamiaceae</subject><subject>Lamiaceae - chemistry</subject><subject>Metabolism</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plant Roots - chemistry</subject><subject>Scutellaria baicalensis</subject><subject>Substrate Specificity</subject><subject>Transferases</subject><subject>Uridine Diphosphate Glucuronic Acid - chemistry</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EokvhEUA-IASHFP9J7DUXhAoUpEqtVHq2ps6kGGXjxRMjlUfhafE2S-HGyZqZ3-cZfR9jT6U4kkKa1xdCaNm4VqmXzr0SonO6UffYSq6tbrQV4j5b3SEH7BHRN1GpzpiH7EAKq1pp7Yr9Oi85DjHAHNPEYep5-AoZwow5_lyaaeCX78-b67GEktMEM77hV1AlI8aJ24af3c0S3YxzhokGzEDIh5w2_CKUGccRcoQ_uoki8RNM-Toe8VCHnAptd-26L5RxLhnpMXswwEj4ZP8essuPH74cf2pOz04-H787bULbqrlBJ0znBqv6NggtUKED7SQ4pwzqTgYBaKTpHShpoatlMNqtg5DOQOh6fcheLP9uc_pekGa_ibQ7CiZMhbwVziqtTQW7BQw5EWUc_DbHDeQbL4XfheJvQ_E7x71z_jYUr6ru2X5Budpg_49qSaECz_cAULVnqA6GSH85tW7t2lXs7YJhdeNHxOwpRJwC9jFjmH2f4n8u-Q0l26wN</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Nagashima, Shigeyuki</creator><creator>Hirotani, Masao</creator><creator>Yoshikawa, Takafumi</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Purification and characterization of UDP-glucuronate: baicalein 7- O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures</title><author>Nagashima, Shigeyuki ; Hirotani, Masao ; Yoshikawa, Takafumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-e90659f72d4c030e2e9a391a9926e351c0ae616d9a217a5c0ac6398c0196ac5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Baicalein</topic><topic>Baicalin</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Chromatography, Gel</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzymes</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Flavanones</topic><topic>Flavonoids - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucuronosyltransferase</topic><topic>Glucuronosyltransferase - chemistry</topic><topic>Glucuronosyltransferase - isolation & purification</topic><topic>Lamiaceae</topic><topic>Lamiaceae - chemistry</topic><topic>Metabolism</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plant Roots - chemistry</topic><topic>Scutellaria baicalensis</topic><topic>Substrate Specificity</topic><topic>Transferases</topic><topic>Uridine Diphosphate Glucuronic Acid - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagashima, Shigeyuki</creatorcontrib><creatorcontrib>Hirotani, Masao</creatorcontrib><creatorcontrib>Yoshikawa, Takafumi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagashima, Shigeyuki</au><au>Hirotani, Masao</au><au>Yoshikawa, Takafumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and characterization of UDP-glucuronate: baicalein 7- O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>53</volume><issue>5</issue><spage>533</spage><epage>538</epage><pages>533-538</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>UDP-glucuronate: baicalein 7-
O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of
Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and chromatofocusing procedures to apparent homogeneity. The
M
r
was estimated to be 110 kDa by gel filtration chromatography with a 52 kDa subunit by SDS-PAGE. The isoelectric point was pH 4.8. UBGAT was specific to UDP-glucuronic acid as a sugar donor and flavones with substitution
ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>10724177</pmid><doi>10.1016/S0031-9422(99)00593-2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9422 |
| ispartof | Phytochemistry (Oxford), 2000-03, Vol.53 (5), p.533-538 |
| issn | 0031-9422 1873-3700 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70972336 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Analytical, structural and metabolic biochemistry Baicalein Baicalin Biological and medical sciences Cell culture Chromatography, Gel Drugs, Chinese Herbal - chemistry Electrophoresis, Polyacrylamide Gel Enzymes Enzymes and enzyme inhibitors Flavanones Flavonoids - chemistry Fundamental and applied biological sciences. Psychology Glucuronosyltransferase Glucuronosyltransferase - chemistry Glucuronosyltransferase - isolation & purification Lamiaceae Lamiaceae - chemistry Metabolism Plant physiology and development Plant Proteins - chemistry Plant Proteins - isolation & purification Plant Roots - chemistry Scutellaria baicalensis Substrate Specificity Transferases Uridine Diphosphate Glucuronic Acid - chemistry |
| title | Purification and characterization of UDP-glucuronate: baicalein 7- O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures |
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