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Liquid chromatography of polymyxin B sulphate
A reversed-phase liquid chromatography method for analysis of polymyxin B sulphate is described. The method uses a YMC-Pack Pro, C 18, 5 μm, 250×4.6 mm I.D. column maintained at 30°C. The mobile phase comprises acetonitrile–sodium sulphate (0.7%, m/v)–phosphoric acid (6.8%, v/v dilution of 85%, m/m...
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Published in: | Journal of Chromatography A 2000-02, Vol.870 (1), p.237-243 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A reversed-phase liquid chromatography method for analysis of polymyxin B sulphate is described. The method uses a YMC-Pack Pro, C
18, 5 μm, 250×4.6 mm I.D. column maintained at 30°C. The mobile phase comprises acetonitrile–sodium sulphate (0.7%, m/v)–phosphoric acid (6.8%, v/v dilution of 85%, m/m phosphoric acid)–water (22.25:50:5:22.75) at a flow-rate of 1.0 ml/min. Detection was by UV at 215 nm. The method is able to resolve polymyxin B
1, the major component, from more than thirty other components present in the complex. Robustness was evaluated by performing a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity. |
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ISSN: | 0021-9673 |
DOI: | 10.1016/S0021-9673(99)00936-X |