Liquid chromatography of polymyxin B sulphate

A reversed-phase liquid chromatography method for analysis of polymyxin B sulphate is described. The method uses a YMC-Pack Pro, C 18, 5 μm, 250×4.6 mm I.D. column maintained at 30°C. The mobile phase comprises acetonitrile–sodium sulphate (0.7%, m/v)–phosphoric acid (6.8%, v/v dilution of 85%, m/m...

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Published in:Journal of Chromatography A 2000-02, Vol.870 (1), p.237-243
Main Authors: Orwa, J.A., Van Gerven, A., Roets, E., Hoogmartens, J.
Format: Article
Language:English
Subjects:
Analysis
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - chemistry
Antibiotics
Biological and medical sciences
Chromatography, Liquid - methods
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Polymyxin B - analysis
Polymyxin B - chemistry
Polymyxins
Protein Conformation
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
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creator Orwa, J.A.
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description A reversed-phase liquid chromatography method for analysis of polymyxin B sulphate is described. The method uses a YMC-Pack Pro, C 18, 5 μm, 250×4.6 mm I.D. column maintained at 30°C. The mobile phase comprises acetonitrile–sodium sulphate (0.7%, m/v)–phosphoric acid (6.8%, v/v dilution of 85%, m/m phosphoric acid)–water (22.25:50:5:22.75) at a flow-rate of 1.0 ml/min. Detection was by UV at 215 nm. The method is able to resolve polymyxin B 1, the major component, from more than thirty other components present in the complex. Robustness was evaluated by performing a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity.
doi_str_mv 10.1016/S0021-9673(99)00936-X
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source Elsevier
subjects Analysis
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - chemistry
Antibiotics
Biological and medical sciences
Chromatography, Liquid - methods
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Polymyxin B - analysis
Polymyxin B - chemistry
Polymyxins
Protein Conformation
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
title Liquid chromatography of polymyxin B sulphate
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