Activation of Peripheral Blood Neutrophils from Patients with Active Advanced Tuberculosis

• Published in: Clinical immunology (Orlando, Fla.) Fla.), 2001-07, Vol.100 (1), p.87-95
• Main Authors: Alemán, Mercedes, Beigier-Bompadre, Macarena, Borghetti, Claudia, de la Barrera, Silvia, Abbate, Eduardo, Isturiz, Martı́n, del C. Sasiain, Marı́a
• Format: Article
• Language: English
• Subjects: AFc receptors; Antigen-Antibody Complex - immunology; Antigens, CD - analysis; Antigens, CD - biosynthesis; Antigens, Differentiation - analysis; Bacterial diseases; Biological and medical sciences; Cell Adhesion Molecules; Chemotaxis, Leukocyte - drug effects; Cytoplasmic Granules - secretion; Cytotoxicity, Immunologic; Fc receptors; Human bacterial diseases; Humans; Immunoglobulin G - immunology; Infectious diseases; Interleukin-1 - biosynthesis; Medical sciences; Mycobacterium tuberculosis; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils - physiology; Receptors, IgG - analysis; Receptors, Tumor Necrosis Factor - biosynthesis; Receptors, Tumor Necrosis Factor, Type I; Respiratory Burst; Superoxides - metabolism; Tuberculosis and atypical mycobacterial infections; Tuberculosis, Pulmonary - blood; Tuberculosis, Pulmonary - immunology; Tumor Necrosis Factor-alpha - biosynthesis; Up-Regulation
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1006/clim.2001.5044

Activation of peripheral blood neutrophils (PMN) was investigated in order to determine whether they might contribute to the inflammatory process during active advanced tuberculosis. Receptors for the Fc portion of IgG (FcγR) (FcγRI, FcγRII, and FcγRIIIB), CD66 (degranulation marker), and receptors for tumor necrosis factor-α (TNF-R55 and TNF-R75) were analyzed on PMN obtained from normal controls and tuberculosis patients (TB-PMN). Functional parameters such as cytotoxicity, superoxide anion generation triggered by N-formyl-methionyl-leucyl-phenyl-alanine (FMLP), and TNF-α and IL-1β production were evaluated. A high expression of TNF-R55, CD66, and FcγRIIIB and the appearance of FcγRI were detected in TB-PMN. In addition, cytotoxicity, superoxide anion release, and TNF-α and IL-1β production were enhanced in TB-PMN. Thus, in tuberculosis, the activation of PMN outside the focus of infection strongly suggests the possibility of a systemic inflammation that could modulate the inflammatory response.