Rapid identification of the ABO genotypes by their single-stand conformation polymorphism

The ABO locus on chromosome 9 contains many more alleles than are currently used routinely in forensic science. The use of singe‐strand conformation polymorphism (SSCP) can separate sequence polymorphisms that differ by only one base. The SSCP process used allows for both single‐ and double‐stranded...

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Bibliographic Details
Published in:Electrophoresis 2000-02, Vol.21 (3), p.537-540
Main Authors: Tsai, Li-Chin, Kao, Li-Gi, Chang, Jan-Gowth, Lee, Hsien-Hsiung, Linacre, Adrian, Lee, James Chun-I
Format: Article
Language:English
Subjects:
ABO Blood-Group System - genetics
ABO genotyping
Alleles
Base Sequence
DNA Primers
Forensic DNA typing
Genotype
Humans
Polymerase chain reaction
Polymorphism, Single-Stranded Conformational
Single-strand conformation polymorphism
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Summary:The ABO locus on chromosome 9 contains many more alleles than are currently used routinely in forensic science. The use of singe‐strand conformation polymorphism (SSCP) can separate sequence polymorphisms that differ by only one base. The SSCP process used allows for both single‐ and double‐stranded polymerase chain reaction (PCR) products to be visualized. The six ABO genotypes can be differentiated by the allele‐specific B and O SSCP patterns. The double‐stranded DNA produced ‘hybrid’ bands due to heterozygous samples and allowed sequence diversity to be detected between alleles of heterozygotes. These ‘hybrid’ bands are valid markers to confirm genotypes of specimens.
ISSN:0173-0835
1522-2683
DOI:10.1002/(SICI)1522-2683(20000201)21:3<537::AID-ELPS537>3.0.CO;2-1