Screening for defects in tryptophan metabolism

We introduced a two-step procedure for the detection of defects in metabolism of tryptophan: (1) HPTLC (described previously) is suitable when starting the investigation, (2) two HPLC methods with isocratic elution and spectrophotometric detection are used at the next step, when pathological finding...

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Bibliographic Details
Published in:Journal of Chromatography A 2000-02, Vol.870 (1), p.289-293
Main Authors: MARKLOVA, E, MAKOVICKOVA, H, KRAKOROVA, I
Format: Article
Language:English
Subjects:
Amino Acid Metabolism, Inborn Errors - diagnosis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer - methods
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Tryptophan
Tryptophan - metabolism
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Summary:We introduced a two-step procedure for the detection of defects in metabolism of tryptophan: (1) HPTLC (described previously) is suitable when starting the investigation, (2) two HPLC methods with isocratic elution and spectrophotometric detection are used at the next step, when pathological findings are to be confirmed and the individual metabolites quantified. The first method enables the assessment of tryptophan, 5-hydroxyindolylacetic acid, indolylacetic acid, indolylacryloylglycine, indolylacrylic acid and its possible precursors, namely indolyllactic and indolylpropionic acids. The second procedure is intended for the monitoring of anthranilic, 3-hydroxyanthranilic, kynurenic and xanthurenic acids, kynurenine, 3-hydroxykynurenine and indoxyl-sulfate. The same pre-treated sample is used for all methods.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00973-5