Isolation and characterization of Dorin M, a lectin from plasma of the soft tick Ornithodoros moubata

A lectin with high hemagglutinating activity, which we have named Dorin M, was identified in the plasma of the soft tick Ornithodoros moubata. The activity of the plasma lectin could be efficiently inhibited by sialic acid, N-acetyl- d-hexosamines and sialoglycoproteins. Dorin M was purified to homo...

Full description

Saved in:
Bibliographic Details
Published in:Insect biochemistry and molecular biology 2000-03, Vol.30 (3), p.195-205
Main Authors: Kovář, Vojtěch, Kopáček, Petr, Grubhoffer, Libor
Format: Article
Language:English
Subjects:
Animals
Argasidae
Hemagglutination Inhibition Tests
Hemagglutination Tests
Lectin
Lectins - chemistry
Lectins - immunology
Lectins - isolation & purification
Mice
Ornithodoros moubata
Sialic acid
Tick plasma
Ticks - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A lectin with high hemagglutinating activity, which we have named Dorin M, was identified in the plasma of the soft tick Ornithodoros moubata. The activity of the plasma lectin could be efficiently inhibited by sialic acid, N-acetyl- d-hexosamines and sialoglycoproteins. Dorin M was purified to homogeneity using two different isolation systems: affinity chromatography on a column of bovine submaxillary mucin conjugated to Sepharose 4B with specific elution by N-acetyl- d-glucosamine and chromatography on Blue-Sepharose followed by anion exchange FPLC on a MonoQ column. The purified lectin is a glycoprotein which, in the native state, forms aggregates with molecular mass of about 640 kDa. Non-reducing SDS PAGE revealed that the lectin consists of two noncovalently bound subunits migrating closely around 37 kDa. Dorin M is a glycoprotein, probably modified by N-type glycosylation. After chemical deglycosylation, only one band of about 32 kDa was detected. Dorin M is the first lectin purified from ticks.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(99)00107-1