Differential Expression of Osteonectin/SPARC during Human Prostate Cancer Progression

The precise mechanism(s) involved in invasion and metastasis of prostate cancer (CaP) is poorly understood. Osteonectin [ON (also known as SPARC or BM-40)] is an antiadhesive protein known to be involved in cell-matrix interactions, migration, and angiogenesis. In this report, we studied the express...

Full description

Saved in:
Bibliographic Details
Published in:Clinical cancer research 2000-03, Vol.6 (3), p.1140-1149
Main Authors: Thomas, R, True, L D, Bassuk, J A, Lange, P H, Vessella, R L
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antibody Specificity
Blotting, Western
Disease Progression
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
In Situ Hybridization
Male
Neoplasm Metastasis
Osteonectin - analysis
Osteonectin - genetics
Osteonectin - immunology
Prostate-Specific Antigen - analysis
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The precise mechanism(s) involved in invasion and metastasis of prostate cancer (CaP) is poorly understood. Osteonectin [ON (also known as SPARC or BM-40)] is an antiadhesive protein known to be involved in cell-matrix interactions, migration, and angiogenesis. In this report, we studied the expression of ON in human prostate cell lines, primary tumors, and metastatic foci of CaP. Reverse transcription-PCR and nonradioactive in situ hybridization (ISH) techniques were used to determine ON gene expression. Immunohistochemistry was carried out using the polyclonal antibody LF37 and/or the monoclonal antibody ON-mAb. Low to moderate levels of ON mRNA and protein were observed in glandular epithelial cells of normal tissue as well as a few primary CaPs. However, high levels of ON mRNA and protein were observed in most of the CaP metastatic foci, both osseous and nonosseous. This correlated well with our findings that multiple different CaP cell lines including four CaP cell lines derived from metastases show high levels of ON gene expression. Furthermore, ISH analyses and cell-specific reverse transcription-PCR evaluation showed that both the luminal and basal cells express the ON gene. We conclude that the differential pattern of ON expression suggests that it may play an important role in the progression of CaP.
ISSN:1078-0432
1557-3265