Ovarian Expression of a Disintegrin and Metalloproteinase with Thrombospondin Motifs During Ovulation in the Gonadotropin-Primed Immature Rat

• Published in: Biology of reproduction 2000-04, Vol.62 (4), p.1090-1095
• Main Authors: Espey, L L, Yoshioka, S, Russell, D L, Robker, R L, Fujii, S, Richards, J S
• Format: Article
• Language: English
• Subjects: ADAM Proteins; ADAMTS1 Protein; Androstenols - pharmacology; Animals; Blotting, Northern; Cyclooxygenase Inhibitors - pharmacology; Disintegrins - genetics; Disintegrins - metabolism; DNA Fragmentation - drug effects; Female; Gonadotropins - pharmacology; In Situ Hybridization; Indomethacin - pharmacology; Metalloendopeptidases - genetics; Metalloendopeptidases - metabolism; Ovary - metabolism; Ovulation - physiology; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Thrombospondins - metabolism
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod62.4.1090

Mammalian ovulation is a dynamic process that requires degradation of the collagenous connective tissue in the thecal layers of a mature follicle. In this reverse transcription-polymerase chain reaction differential display study, gonadotropin-primed immature rats were used to detect ovarian expression of a relatively new type of disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-1) that is known to cleave extracellular matrix in acutely inflamed tissues. Immature Wistar rats were primed with 10 IU eCG s.c., and the temporal pattern of expression of the ADAMTS-1 gene was delineated by extracting ovarian RNA at 0, 2, 4, 8, 12, and 24 h after induction of ovulation by injecting the primed animals with 10 IU hCG s.c. The differential display data, Northern analyses, and in situ hybridization micrographs all showed significant up-regulation of ADAMTS-1 gene expression by 8 h after hCG administration. The in situ data indicated that the ADAMTS-1 mRNA was in the granulosa layer of mature follicles. Expression reached a peak at 12 h and remained elevated at 24 h after hCG. ADAMTS-1 gene expression was impaired by the antiprogesterone agent epostane, but this inhibition could be overcome by exogenous progesterone. ADAMTS-1 expression was not affected when ovulation was blocked by treatment of the animals with the anti-eicosanoid agent indomethacin. In conclusion, the temporal pattern of expression of this gene, and its apparent regulation by progesterone, suggests that ADAMTS-1 has a significant role in the inflammatory events of the ovulatory process.