Elevated tissue factor levels in leukemic cell homogenate

Tissue factor (TF) antigen and activity were measured in leukemic cell homogenates. In leukemic cell homogenate, especially that of acute promyelocytic leukemia (APL), both TF antigen and activity were significantly higher than these levels in the mononuclear cells obtained from healthy volunteers....

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Bibliographic Details
Published in:Clinical and applied thrombosis/hemostasis 2000-01, Vol.6 (1), p.14-17
Main Authors: Nakasaki, T, Wada, H, Watanabe, R, Mori, Y, Gabazza, E C, Kageyama, S, Nishikawa, M, Shiku, H
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - blood
Disseminated Intravascular Coagulation - blood
Disseminated Intravascular Coagulation - etiology
Humans
Leukemia - complications
Leukemia - pathology
Leukemia, Lymphocytic, Chronic, B-Cell - pathology
Leukemia, Monocytic, Acute - pathology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology
Leukemia, Myelomonocytic, Acute - pathology
Leukemia, Promyelocytic, Acute - pathology
Leukemia, T-Cell - pathology
Leukocytes, Mononuclear - chemistry
Neoplasm Proteins - blood
Neoplasm Proteins - immunology
Neoplasm Proteins - metabolism
Thromboplastin - immunology
Thromboplastin - metabolism
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Summary:Tissue factor (TF) antigen and activity were measured in leukemic cell homogenates. In leukemic cell homogenate, especially that of acute promyelocytic leukemia (APL), both TF antigen and activity were significantly higher than these levels in the mononuclear cells obtained from healthy volunteers. Both TF antigen and activity were significantly higher in myelocytic leukemia than in lymphocytic leukemia cells. In leukemic cell homogenates, there was a close correlation between TF antigen and TF activity. The TF activity/TF antigen ratio was significantly higher in myelocytic leukemia than in lymphocytic leukemia cells. As the TF activity was not increased in lymphocytic leukemia cell homogenates to which were added phospholipids, the decrease in TF activity in lymphocytic leukemia might not be due to phospholipid in the leukemic cell membrane. Values for TF activity, TF antigen, and the TF activity/TF antigen ratio in leukemic cell homogenate from patients with disseminated intravascular coagulation (DIC) were significantly higher than those in patients without DIC. Therefore, the measurement of TF antigen and activity in leukemic cells could be useful for the prediction of DIC.
ISSN:1076-0296
DOI:10.1177/107602960000600102