Identification of DNA Components Required for Induction of Cotton Leaf Curl Disease

Cotton leaf curl disease (CLCuD) is a major constraint to cotton production in Pakistan. Infectious clones of the monopartite begomovirus cotton leaf curl virus (CLCuV), associated with diseased cotton, are unable to induce typical symptoms in host plants. We have identified and isolated a single-st...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2001-07, Vol.285 (2), p.234-243
Main Authors: Briddon, R.W., Mansoor, S., Bedford, I.D., Pinner, M.S., Saunders, K., Stanley, J., Zafar, Y., Malik, K.A., Markham, P.G.
Format: Article
Language:English
Subjects:
Base Sequence
Begomovirus
cotton
Cotton leaf curl virus
DNA ^b
DNA β
DNA, Circular
DNA, Viral - physiology
evolution
Geminiviridae - genetics
Geminiviridae - physiology
geminivirus
Gossypium - virology
Molecular Sequence Data
Pakistan
Plant Diseases - virology
recombination
Sequence Analysis, DNA
whitefly
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Summary:Cotton leaf curl disease (CLCuD) is a major constraint to cotton production in Pakistan. Infectious clones of the monopartite begomovirus cotton leaf curl virus (CLCuV), associated with diseased cotton, are unable to induce typical symptoms in host plants. We have identified and isolated a single-stranded DNA molecule approximately 1350 nucleotides in length which, when coinoculated with the begomovirus to cotton, induces symptoms typical of CLCuD, including vein swelling, vein darkening, leaf curling, and enations. This molecule (termed DNA β) requires the begomovirus for replication and encapsidation. The CLCuV/DNA 1/DNA β complex, together with a similar complex previously identified in Ageratum conyzoides, represent members of an entirely new type of infectious, disease-causing agents. The implications of this finding to our understanding of the evolution of new disease-causing agents are discussed.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.0949