A Micromethod to Measure Platelet Aggregation and ATP Release by Impedance

A standard electrode has been modified in order to detect platelet aggregation by impedance in small volumes of whole blood. The modified electrode was constructed to fit into a standard 0.5-ml cuvette normally employed for detecting platelet aggregation in platelet-rich plasma (PRP) on a Chrono-log...

Full description

Saved in:
Bibliographic Details
Published in:Thrombosis research 2001-07, Vol.103 (1), p.57-61
Main Authors: Goldenberg, Seth J, Veriabo, Nicholas J, Soslau, Gerald
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - analysis
Adenosine Triphosphate - metabolism
Aggregometer
ATP
Biological and medical sciences
Electric Impedance
Firefly Luciferin
Hematology
Humans
Impedance
Investigative techniques, diagnostic techniques (general aspects)
Luminescent Measurements
Medical sciences
Methods
Microchemistry
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Platelet aggregation
Platelet Aggregation - drug effects
Platelet Function Tests - methods
Platelet Function Tests - standards
Reproducibility of Results
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A standard electrode has been modified in order to detect platelet aggregation by impedance in small volumes of whole blood. The modified electrode was constructed to fit into a standard 0.5-ml cuvette normally employed for detecting platelet aggregation in platelet-rich plasma (PRP) on a Chrono-log Aggregometer (Haverford, PA). Whole blood samples were mixed 1:1 with saline and total volumes of 1 ml, 500 μl and 300 μl were tested. ATP release was recorded concurrently with aggregation by measuring the luminescence from the firefly luciferin–luciferase reaction. The modified electrode could detect aggregation in the 300- and 500-μl samples with the same efficiency as the 1-ml samples. ATP release in the 300- and 500-μl samples yielded equivalent levels of release as detected with the 1-ml samples. This electrode is well suited for evaluation of platelet function in research and clinical blood samples where only small volumes of blood are available.
ISSN:0049-3848
1879-2472
DOI:10.1016/S0049-3848(01)00269-9