Summary of workshop findings for porcine myelomonocytic markers

About 65 monoclonal antibodies (mAb) including 17 internal controls were analyzed for their ability to recognize and bind to various cells of the myelomonocytic lineage. Flow cytometry (FCM) utilizing both single and double staining, and immunoprecipitation (IP) assays were used in the analysis. Abo...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2001-07, Vol.80 (1), p.93-109
Main Authors: Thacker, E., Summerfield, A., McCullough, K., Ezquerra, A., Dominguez, J., Alonso, F., Lunney, J., Sinkora, J., Haverson, K.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigens, CD
Antigens, Differentiation, Myelomonocytic
CD11
CD14
CD163
CD92
CD93
Cluster Analysis
Cluster of differentiation (CD) antigens
Flow Cytometry
Lipopolysaccharide Receptors
Monoclonal antibodies (mAb)
Monocytes - classification
Monocytes - cytology
Monocytes - immunology
Myeloid cells
Pig
SWC3
SWC8
Swine - immunology
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Summary:About 65 monoclonal antibodies (mAb) including 17 internal controls were analyzed for their ability to recognize and bind to various cells of the myelomonocytic lineage. Flow cytometry (FCM) utilizing both single and double staining, and immunoprecipitation (IP) assays were used in the analysis. About 38 of the mAb were reactive with myelomonocytic cells, resulting in nine clusters of interest. Although the exact identity of many of the molecules on the cells bound by the mAb remains undetermined, information obtained about the mAb analyzed in this workshop should be helpful in further identifying various populations of myelomonocytic cells and their stages of differentiation. Out of 12 mAbs with potential CD11 specificity, seven were assigned to three different swine specific alpha chains of the CD11/CD18 integrin heterodimer, the assignment of the remaining four was tentative. One antibody had a binding specificity consistent with SWC3 and one with SWC8. CD14 expression on pig cells was characterized with a panel of CD14-positive antibodies, two of these antibodies were assigned to swine CD14. Two antibodies were assigned to CD163. Further work is required to determine the antigens recognized by many of the other mAb.
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(01)00278-1