Interaction of cyanine dyes with nucleic acids. XXV. Influence of affinity-modifying groups in the structure of benzothiazol-4-[2,6-dimethylpyridinium] dyes on the spectral properties of the dyes in the presence of nucleic acids

Novel monomethine pyridinium cyanine dyes of similar structure and containing ‘affinity-modifying’ groups of different chemical nature were studied by spectral-luminescent methods as possible fluorescent probes for the nucleic acids detection. It was shown that the nature of the functional groups in...

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Bibliographic Details
Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2001-06, Vol.57 (7), p.1533-1540
Main Authors: Yarmoluk, S.M, Kovalska, V.B, Kryvorotenko, D.V, Balanda, A.O, Ogul'chansky, T.Yu
Format: Article
Language:English
Subjects:
Complexation
DNA - chemistry
Fluorescence
Fluorescent detection
Fluorescent Dyes - chemistry
Luminescence
Monomethine cyanine dyes
Nucleic acids
Nucleic Acids - chemistry
Spectrometry, Fluorescence
Structure-Activity Relationship
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Summary:Novel monomethine pyridinium cyanine dyes of similar structure and containing ‘affinity-modifying’ groups of different chemical nature were studied by spectral-luminescent methods as possible fluorescent probes for the nucleic acids detection. It was shown that the nature of the functional groups in the dye linker influences the fluorescent properties of the dye–nucleic acids complexes. Incorporation of a hydroxyl group into the linker structure leads to a significant increase in the fluorescence intensity of the dye — double-stranded DNA complexes relative to the parent dye Cyan 40.
ISSN:1386-1425
DOI:10.1016/S1386-1425(01)00435-8