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Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry
Objectives: The aim of the current study was to develop a simple, fast and universal method for quantification of any combination of the three major immunosuppressants sirolimus, tacrolimus and cyclosporin in whole blood, using a LC-tandem mass spectrometer (API-2000, SCIEX, Toronto, Canada). Method...
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Published in: | Clinical biochemistry 2001-06, Vol.34 (4), p.285-290 |
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container_title | Clinical biochemistry |
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creator | Volosov, Andrew Napoli, Kimberly L. Soldin, Steven J. |
description | Objectives: The aim of the current study was to develop a simple, fast and universal method for quantification of any combination of the three major immunosuppressants sirolimus, tacrolimus and cyclosporin in whole blood, using a LC-tandem mass spectrometer (API-2000, SCIEX, Toronto, Canada).
Methods: 250 μL whole blood was spiked with internal standard (ritonavir), and protein precipitated with 350 μL acetonitrile. The sample was centrifuged and 30 μL aliquot was injected onto the HPLC column, where it underwent an online extraction with ammonium acetate. After that the automatic switching valve was activated, changing the mobile phase to methanol and thereby eluting the analytes into the tandem mass spectrometer. The high selectivity of a tandem mass analyzer allows determination of any combination of the three drugs within a 5 min run.
Results: Between-day precision was between 2.4% and 9.7% for all analytes at the concentrations tested. Accuracy ranged between 98.8% and 103.2% (
n = 20). The method was linear over the measuring ranges of all analytes. Within-run precision was below %CV = 6% for all analytes. Good correlation with other analytical methods was observed.
Conclusions: The simplicity, universality and high throughput of the method make it suitable for application in a clinical laboratory. The method has been implemented in our laboratory for a routine use. |
doi_str_mv | 10.1016/S0009-9120(01)00235-1 |
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Methods: 250 μL whole blood was spiked with internal standard (ritonavir), and protein precipitated with 350 μL acetonitrile. The sample was centrifuged and 30 μL aliquot was injected onto the HPLC column, where it underwent an online extraction with ammonium acetate. After that the automatic switching valve was activated, changing the mobile phase to methanol and thereby eluting the analytes into the tandem mass spectrometer. The high selectivity of a tandem mass analyzer allows determination of any combination of the three drugs within a 5 min run.
Results: Between-day precision was between 2.4% and 9.7% for all analytes at the concentrations tested. Accuracy ranged between 98.8% and 103.2% (
n = 20). The method was linear over the measuring ranges of all analytes. Within-run precision was below %CV = 6% for all analytes. Good correlation with other analytical methods was observed.
Conclusions: The simplicity, universality and high throughput of the method make it suitable for application in a clinical laboratory. The method has been implemented in our laboratory for a routine use.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/S0009-9120(01)00235-1</identifier><identifier>PMID: 11440728</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Calibration ; Chemistry, Clinical - methods ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Cyclosporin ; Cyclosporine - blood ; Cyclosporine - isolation & purification ; Gas Chromatography-Mass Spectrometry - methods ; Humans ; Immunosuppressants ; Immunosuppressive Agents - blood ; Immunosuppressive Agents - chemistry ; Immunosuppressive Agents - isolation & purification ; Linear Models ; Reproducibility of Results ; Sensitivity and Specificity ; Sirolimus ; Sirolimus - blood ; Sirolimus - isolation & purification ; Tacrolimus ; Tacrolimus - blood ; Tacrolimus - isolation & purification ; Tandem mass-spectrometry ; Transplantations</subject><ispartof>Clinical biochemistry, 2001-06, Vol.34 (4), p.285-290</ispartof><rights>2001 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-fd5f7290c45e18ec3595270b242ab1e99ce398422e846be348bb560c2b72ebcf3</citedby><cites>FETCH-LOGICAL-c361t-fd5f7290c45e18ec3595270b242ab1e99ce398422e846be348bb560c2b72ebcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11440728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Volosov, Andrew</creatorcontrib><creatorcontrib>Napoli, Kimberly L.</creatorcontrib><creatorcontrib>Soldin, Steven J.</creatorcontrib><title>Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>Objectives: The aim of the current study was to develop a simple, fast and universal method for quantification of any combination of the three major immunosuppressants sirolimus, tacrolimus and cyclosporin in whole blood, using a LC-tandem mass spectrometer (API-2000, SCIEX, Toronto, Canada).
Methods: 250 μL whole blood was spiked with internal standard (ritonavir), and protein precipitated with 350 μL acetonitrile. The sample was centrifuged and 30 μL aliquot was injected onto the HPLC column, where it underwent an online extraction with ammonium acetate. After that the automatic switching valve was activated, changing the mobile phase to methanol and thereby eluting the analytes into the tandem mass spectrometer. The high selectivity of a tandem mass analyzer allows determination of any combination of the three drugs within a 5 min run.
Results: Between-day precision was between 2.4% and 9.7% for all analytes at the concentrations tested. Accuracy ranged between 98.8% and 103.2% (
n = 20). The method was linear over the measuring ranges of all analytes. Within-run precision was below %CV = 6% for all analytes. Good correlation with other analytical methods was observed.
Conclusions: The simplicity, universality and high throughput of the method make it suitable for application in a clinical laboratory. The method has been implemented in our laboratory for a routine use.</description><subject>Calibration</subject><subject>Chemistry, Clinical - methods</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cyclosporin</subject><subject>Cyclosporine - blood</subject><subject>Cyclosporine - isolation & purification</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Humans</subject><subject>Immunosuppressants</subject><subject>Immunosuppressive Agents - blood</subject><subject>Immunosuppressive Agents - chemistry</subject><subject>Immunosuppressive Agents - isolation & purification</subject><subject>Linear Models</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Sirolimus</subject><subject>Sirolimus - blood</subject><subject>Sirolimus - isolation & purification</subject><subject>Tacrolimus</subject><subject>Tacrolimus - blood</subject><subject>Tacrolimus - isolation & purification</subject><subject>Tandem mass-spectrometry</subject><subject>Transplantations</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkMtuFDEQRS0EIpPAJ4C8QmTR4Ee_vEIo4iVFYhFYW7a7mnHUbve43Eizg3_IF-ZLcDIjsszKKvncuqpDyCvO3nHG2_dXjDFVKS7YW8bPGROyqfgTsuF9JyuhpHxKNv-RE3KKeF1GUfftc3LCeV2zTvQb8vfKh3XKZoa4IkUflgmomQc6Gsx0t5o5-9E7k32caRxp3iYAGsx1TNSHsM4R12VJgFhIpHZPJ79b_UDdNsVgcvyVzLLd3_65KR0DhBJFrHABl8s_5LR_QZ6NZkJ4eXzPyM_Pn35cfK0uv3_5dvHxsnKy5bkah2bshGKuboD34GSjGtExK2phLAelHEjV10JAX7cWZN1b27TMCdsJsG6UZ-TNYe-S4m4FzDp4dDBNh9t1x5TiSnUFbA6gSxExwaiX5INJe82ZvnOv793rO7GacX3vXvOSe30sWG2A4SF1lF2ADwcAypm_PSSNzsPsYPCp-NBD9I9U_AOVopjF</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Volosov, Andrew</creator><creator>Napoli, Kimberly L.</creator><creator>Soldin, Steven J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry</title><author>Volosov, Andrew ; Napoli, Kimberly L. ; Soldin, Steven J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-fd5f7290c45e18ec3595270b242ab1e99ce398422e846be348bb560c2b72ebcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Calibration</topic><topic>Chemistry, Clinical - methods</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cyclosporin</topic><topic>Cyclosporine - blood</topic><topic>Cyclosporine - isolation & purification</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Humans</topic><topic>Immunosuppressants</topic><topic>Immunosuppressive Agents - blood</topic><topic>Immunosuppressive Agents - chemistry</topic><topic>Immunosuppressive Agents - isolation & purification</topic><topic>Linear Models</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Sirolimus</topic><topic>Sirolimus - blood</topic><topic>Sirolimus - isolation & purification</topic><topic>Tacrolimus</topic><topic>Tacrolimus - blood</topic><topic>Tacrolimus - isolation & purification</topic><topic>Tandem mass-spectrometry</topic><topic>Transplantations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Volosov, Andrew</creatorcontrib><creatorcontrib>Napoli, Kimberly L.</creatorcontrib><creatorcontrib>Soldin, Steven J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Volosov, Andrew</au><au>Napoli, Kimberly L.</au><au>Soldin, Steven J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>34</volume><issue>4</issue><spage>285</spage><epage>290</epage><pages>285-290</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>Objectives: The aim of the current study was to develop a simple, fast and universal method for quantification of any combination of the three major immunosuppressants sirolimus, tacrolimus and cyclosporin in whole blood, using a LC-tandem mass spectrometer (API-2000, SCIEX, Toronto, Canada).
Methods: 250 μL whole blood was spiked with internal standard (ritonavir), and protein precipitated with 350 μL acetonitrile. The sample was centrifuged and 30 μL aliquot was injected onto the HPLC column, where it underwent an online extraction with ammonium acetate. After that the automatic switching valve was activated, changing the mobile phase to methanol and thereby eluting the analytes into the tandem mass spectrometer. The high selectivity of a tandem mass analyzer allows determination of any combination of the three drugs within a 5 min run.
Results: Between-day precision was between 2.4% and 9.7% for all analytes at the concentrations tested. Accuracy ranged between 98.8% and 103.2% (
n = 20). The method was linear over the measuring ranges of all analytes. Within-run precision was below %CV = 6% for all analytes. Good correlation with other analytical methods was observed.
Conclusions: The simplicity, universality and high throughput of the method make it suitable for application in a clinical laboratory. The method has been implemented in our laboratory for a routine use.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11440728</pmid><doi>10.1016/S0009-9120(01)00235-1</doi><tpages>6</tpages></addata></record> |
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subjects | Calibration Chemistry, Clinical - methods Chromatography Chromatography, High Pressure Liquid - methods Cyclosporin Cyclosporine - blood Cyclosporine - isolation & purification Gas Chromatography-Mass Spectrometry - methods Humans Immunosuppressants Immunosuppressive Agents - blood Immunosuppressive Agents - chemistry Immunosuppressive Agents - isolation & purification Linear Models Reproducibility of Results Sensitivity and Specificity Sirolimus Sirolimus - blood Sirolimus - isolation & purification Tacrolimus Tacrolimus - blood Tacrolimus - isolation & purification Tandem mass-spectrometry Transplantations |
title | Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry |
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