Diagnosis of sporotrichosis in a donkey using direct fluorescein-labeled antibody testing

Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA. A 4-year-old female donkey residing in an open field in Indiana was admitted for evaluation of facial lesions of 2 years duration. Cytologic and histologic examination of exudate a...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2000-03, Vol.12 (2), p.180-183
Main Authors: Irizarry-Rovira, A.R, Kaufman, L, Christian, J.A, Reberg, S.R, Adams, S.B, DeNicola, D.B, Rivers, W, Hawkins, J.F
Format: Article
Language:English
Subjects:
Animal mycoses
Animals
antibody detection
asses
Biological and medical sciences
case studies
Diagnosis, Differential
disease diagnosis
Equidae - microbiology
Female
fluorescent antibody technique
Fluorescent Antibody Technique, Direct - veterinary
Infectious diseases
lesions
Medical sciences
Mycoses
Sporothrix - immunology
Sporothrix schenckii
sporotrichosis
Sporotrichosis - diagnosis
Sporotrichosis - immunology
Sporotrichosis - veterinary
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Summary:Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA. A 4-year-old female donkey residing in an open field in Indiana was admitted for evaluation of facial lesions of 2 years duration. Cytologic and histologic examination of exudate and tissue from the lesions revealed a pyogranulomatous inflammatory reaction with numerous yeasts. Sporothrix schenckii was suspected to be the infectious agent; however, multiple culture attempts did not provide positive identification of the organism. Serologic examination supported infection with S. schenckii. A specific direct immunofluorescent antibody test performed on paraffin-embedded tissue sections confirmed the organism as S. schenckii. Clinical signs resolved after appropriate iodide therapy.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870001200218