Establishment and characterization of macrophage-like cell lines expressing osteoclast-specific markers

Osteoclasts differentiate from hematopoietic precursors of the monocyte/macrophage lineage to mononuclear preosteoclasts and multinuclear mature osteoclasts. In the present study, we report on the establishment of macrophage like cell lines from mouse bone marrow by coculturing bone marrow cells wit...

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Bibliographic Details
Published in:Journal of bone and mineral metabolism 2001, Vol.19 (4), p.220-227
Main Authors: SAKIYAMA, Hisako, MASUDA, Riako, INOUE, Naokazu, YAMAMOTO, Katusi, KURIIWA, Kazuko, NAKAGAWA, Koichi, YOSHIDA, Kazuko
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins
Base Sequence
Biological and medical sciences
Biomarkers
Bone marrow
Cell Differentiation
Cell Line
Cells
Coculture Techniques
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression
Glycoproteins - genetics
Immunohistochemistry
Macrophages - cytology
Macrophages - metabolism
Matrix Metalloproteinase 9 - genetics
Medical research
Mice
Microscopy, Electron, Scanning
Osteoclasts - cytology
Osteoclasts - metabolism
Osteoprotegerin
Receptors, Calcitonin - genetics
Receptors, Cytoplasmic and Nuclear - genetics
Receptors, Tumor Necrosis Factor
Receptors, Vitronectin - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA-Binding Proteins - genetics
Skeleton and joints
Transcription Factors - genetics
Vacuolar Proton-Translocating ATPases - metabolism
Vertebrates: osteoarticular system, musculoskeletal system
Citations: Items that cite this one
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Summary:Osteoclasts differentiate from hematopoietic precursors of the monocyte/macrophage lineage to mononuclear preosteoclasts and multinuclear mature osteoclasts. In the present study, we report on the establishment of macrophage like cell lines from mouse bone marrow by coculturing bone marrow cells with mouse chondrocytes. Isolated clones (MLC-6 and MLC-7 cells) expressed fully differentiated osteoclast markers, such as calcitonin receptors, vitronectin receptors, tartrate-resistant acid phosphatase and vacuolar H+ -ATPase, in the absence of osteoclast differentiation factor/osteoprotegerin ligand/RANKL/TRANCE, which was essential for osteoclast differentiation. Most clones also maintained expression of a macrophage-associated protein, namely non-specific esterase. Both MLC-6 and MLC-7 cells released cathepsin K into the culture medium. Both clones resolved dentine slices when cocultured with the osteoblast cell line ST2. The cloned cell lines are considered to be useful tools in the study of osteoclast differentiation.
ISSN:0914-8779
1435-5604
DOI:10.1007/s007740170024