Dynamics of nuclei of muscle fibers and connective tissue cells in normal and denervated rat muscles

The mitotic activity in muscles of growing rats and the effect of denervation were studied by means of continuous infusion of 5‐bromo‐2‐deoxyuridine (BRDU). Denervated muscles after 10 weeks contained 20 to 60% fewer muscle nuclei than normal; BRDU labeled about 25% of the nuclei of normal soleus an...

Full description

Saved in:
Bibliographic Details
Published in:Muscle & nerve 2000-04, Vol.23 (4), p.617-626
Main Authors: Schmalbruch, H., Lewis, D.M.
Format: Article
Language:English
Subjects:
Animals
Atrophy
Biological and medical sciences
BRDU
Bromodeoxyuridine
Cell Differentiation
Cell Nucleus - ultrastructure
Connective Tissue - innervation
Connective Tissue Cells - cytology
Connective Tissue Cells - ultrastructure
denervation
Electric Stimulation
Fundamental and applied biological sciences. Psychology
growth
Isometric Contraction - physiology
Male
Mitosis
Muscle Denervation
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - ultrastructure
Muscle, Skeletal - cytology
Muscle, Skeletal - innervation
Muscle, Skeletal - ultrastructure
nuclei
rat
Rats
Rats, Wistar
skeletal muscle
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mitotic activity in muscles of growing rats and the effect of denervation were studied by means of continuous infusion of 5‐bromo‐2‐deoxyuridine (BRDU). Denervated muscles after 10 weeks contained 20 to 60% fewer muscle nuclei than normal; BRDU labeled about 25% of the nuclei of normal soleus and extensor digitorum longus (EDL) and of denervated EDL muscles but only 5% in the denervated soleus muscle. Labeled nuclei persisted in denervated but not in normal muscles. After the main growth period, the turnover of myonuclei was at most 1 to 2% per week. The behavior of connective tissue nuclei was similar to that in muscle fibers. Infusion of BRDU had no effect on contractile properties. It is suggested that the exceptionally rapid atrophy of the denervated rat soleus associated with loss of satellite cells was due to loss of myonuclei and differentiation and fusion of satellite cells. The cause may possibly be that the phase of postdenervation fibrillation is shorter than in other muscles. © 2000 John Wiley & Sons, Inc. Muscle Nerve 23: 617–626, 2000.
ISSN:0148-639X
1097-4598
DOI:10.1002/(SICI)1097-4598(200004)23:4<617::AID-MUS22>3.0.CO;2-Y