Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica

• Published in: Veterinary parasitology 2000-03, Vol.89 (1), p.153-161
• Main Authors: Duménigo, B.E., Espino, A.M., Finlay, C.M., Mezo, M.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - blood; Antibodies, Monoclonal - immunology; Antigens, Helminth - analysis; Antigens, Helminth - blood; Antigens, Helminth - immunology; Cattle: Trematoda; Circulating antigen; Coproantigen; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; ES antigen detection; Fasciola hepatica; Fasciola hepatica - immunology; Fasciola hepatica - isolation & purification; Fascioliasis - diagnosis; Fascioliasis - immunology; Fascioliasis - veterinary; Feces - parasitology; Female; Kinetics; Kinetics, sandwich ELISA; Parasite Egg Count - veterinary; Sheep; Sheep Diseases - diagnosis; Sheep Diseases - immunology; Sheep Diseases - parasitology
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/S0304-4017(00)00206-5

The monoclonal antibody ES78 was used in a sandwich immunosorbent assay (Sandwich ELISA) for the detection of antigens in sera and faeces in the course of Fasciola hepatica infection in 10 experimentally infected sheep.. All infected sheep had circulating antigens in the first week post-infection (WPI). Antigenemia was detectable until WPI 3 in four infected sheep, WPI 4 in five infected sheep and in only one sheep by WPI 5. The detection of coproantigens (Fa g) was possible in five infected sheep at WPI-4, in four sheep at WPI-5 and in one sheep only at WPI-6. This technique was compared to an indirect ELISA for the detection of antibodies using excretory secretory antigens of F. hepatica. A significant correlation was found between Fa g and egg output and also with adult worm numbers. Our method demonstrated that the diagnosis of active fasciolosis in sheep is possible during all periods of infection.