Endurance training, expression, and physiology of LDH, MCT1, and MCT4 in human skeletal muscle

1  Department of Integrative Biology, University of California, Berkeley 94720; 3  Palo Alto Veterans Affairs Health Care System, Palo Alto, California 94304; 4  Division of Cardiology, University of Colorado Health Sciences Center, Denver, Colorado 80262; and 2  Université Joseph Fourier, Grenoble,...

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Published in:American journal of physiology: endocrinology and metabolism 2000-04, Vol.278 (4), p.E571-E579
Main Authors: Dubouchaud, Herve, Butterfield, Gail E, Wolfel, Eugene E, Bergman, Bryan C, Brooks, George A
Format: Article
Language:English
Subjects:
Adult
Amino Acid Sequence
Anaerobic Threshold - physiology
Blotting, Western
Carrier Proteins - metabolism
Carrier Proteins - physiology
Humans
Isoenzymes
L-Lactate Dehydrogenase - metabolism
L-Lactate Dehydrogenase - physiology
Lactic Acid - metabolism
Male
Mitochondria, Muscle - enzymology
Mitochondria, Muscle - metabolism
Molecular Sequence Data
Monocarboxylic Acid Transporters
Muscle Proteins
Muscle, Skeletal - enzymology
Muscle, Skeletal - physiology
Myosin Heavy Chains - metabolism
Oxidation-Reduction
Physical Endurance - physiology
Physical Fitness - physiology
Prostaglandin-Endoperoxide Synthases - biosynthesis
Subcellular Fractions - metabolism
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Summary:1  Department of Integrative Biology, University of California, Berkeley 94720; 3  Palo Alto Veterans Affairs Health Care System, Palo Alto, California 94304; 4  Division of Cardiology, University of Colorado Health Sciences Center, Denver, Colorado 80262; and 2  Université Joseph Fourier, Grenoble, France To evaluate the effects of endurance training on the expression of monocarboxylate transporters (MCT) in human vastus lateralis muscle, we compared the amounts of MCT1 and MCT4 in total muscle preparations (MU) and sarcolemma-enriched (SL) and mitochondria-enriched (MI) fractions before and after training. To determine if changes in muscle lactate release and oxidation were associated with training-induced changes in MCT expression, we correlated band densities in Western blots to lactate kinetics determined in vivo. Nine weeks of leg cycle endurance training [75% peak oxygen consumption ( O 2 peak )] increased muscle citrate synthase activity (+75%, P  
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.2000.278.4.e571