Human cytomegalovirus induces apoptosis in the hematopoietic cell line MO7e

Several studies have shown that human cytomegalovirus (HCMV) induces growth suppression of hematopoietic progenitors. In vitro studies have demonstrated that the HCMV‐induced suppression is independent of viral protein production. Previous studies have indicated a link between HCMV infection and apo...

Full description

Saved in:
Bibliographic Details
Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2000-01, Vol.108 (3), p.223-230
Main Authors: Sindre, Hilde, Rollag, Halvor, Olafsen, Mette KløVstad, Degre, Miklos, Hestdal, Kjetil
Format: Article
Language:English
Subjects:
Apoptosis
Biological and medical sciences
Cell Division
Cell Line
Cytomegalovirus - physiology
DNA Fragmentation
Fundamental and applied biological sciences. Psychology
hematopoietic cell line
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - virology
Human cytomegalovirus
Humans
Microbiology
Phosphatidylserines - metabolism
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Several studies have shown that human cytomegalovirus (HCMV) induces growth suppression of hematopoietic progenitors. In vitro studies have demonstrated that the HCMV‐induced suppression is independent of viral protein production. Previous studies have indicated a link between HCMV infection and apoptosis in human cells. The purpose of our study was to investigate whether the observed inhibitory effect of HCMV on the human myeloid progenitors could be connected to the induction of apoptosis. The growth and cell death of the hematopoietic cell line MO7e was investigated following infection with HCMV virions and dense bodies. Both virions and dense bodies inhibited the growth of MO7e cells, and induced cell death measured by trypan blue staining. In addition, both HCMV virions and dense bodies caused an increased amount of apoptosis‐characteristic DNA fragmentation in the MO7e cells compared to mock‐treated cells. The HCMV virions were also able to induce an increased expression of phosphatidylserine on the cell surface, which is an early event in the initiation of apoptosis in most cell types. In conclusion, HCMV and HCMV dense bodies are able to induce apoptosis in the myeloid progenitor cell line MO7e
ISSN:0903-4641
1600-0463
DOI:10.1034/j.1600-0463.2000.d01-48.x