Characterization of Human Skeletal Muscle Ankrd2

Human Ankrd2 transcript encodes a 37-kDa protein that is similar to mouse Ankrd2 recently shown to be involved in hypertrophy of skeletal muscle. These novel ankyrin-rich proteins are related to C-193/CARP/MARP, a cardiac protein involved in the control of cardiac hypertrophy. A human genomic region...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-07, Vol.285 (2), p.378-386
Main Authors: Pallavicini, Alberto, Kojić, Snezana, Bean, Camilla, Vainzof, Mariz, Salamon, Michela, Ievolella, Chiara, Bortoletto, Gladis, Pacchioni, Beniamima, Zatz, Mayana, Lanfranchi, Gerolamo, Faulkner, Georgine, Valle, Giorgio
Format: Article
Language:English
Subjects:
Animals
Ankrd2 gene
ankyrin
Ankyrin Repeat
Base Sequence
Binding Sites
Cells, Cultured
Female
Humans
hypertrophy
Male
Mice
Molecular Sequence Data
Molecular Weight
muscle plasticity
muscle proteins
Muscle Proteins - analysis
Muscle Proteins - genetics
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Muscular Dystrophies - genetics
Muscular Dystrophies - metabolism
Muscular Dystrophies - pathology
Nuclear Proteins
Organ Specificity
Promoter Regions, Genetic
Repressor Proteins
RNA, Messenger - analysis
RNA, Messenger - genetics
Sequence Alignment
Sequence Homology, Nucleic Acid
signaling pathways
TATA Box
Transcription Factors - metabolism
Transcription, Genetic
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Summary:Human Ankrd2 transcript encodes a 37-kDa protein that is similar to mouse Ankrd2 recently shown to be involved in hypertrophy of skeletal muscle. These novel ankyrin-rich proteins are related to C-193/CARP/MARP, a cardiac protein involved in the control of cardiac hypertrophy. A human genomic region of 14,300 bp was sequenced revealing a gene organization similar to mouse Ankrd2 with nine exons, four of which encode ankyrin repeats. The intracellular localization of Ankrd2 was unknown since no protein studies had been reported. In this paper we studied the intracellular localization of the protein and its expression on differentiation using polyclonal and monoclonal antibodies produced to human Ankrd2. In adult skeletal muscle Ankrd2 is found in slow fibers; however, not all of the slow fibers express Ankrd2 at the same level. This is particularly evident in dystrophic muscles, where the expression of Ankrd2 in slow fibers seems to be severely reduced.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5131