Single-fiber myosin heavy chain polymorphism during postnatal development: modulation by hypothyroidism

Department of Physiology and Biophysics and Orthopaedics, College of Medicine, University of California, Irvine, California 92697 The primary objective of this study was to follow the developmental time course of myosin heavy chain (MHC) isoform transitions in single fibers of the rodent plantaris m...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2000-04, Vol.278 (4), p.1099-R1106
Main Authors: di Maso, Nick A, Caiozzo, Vincent J, Baldwin, Kenneth M
Format: Article
Language:English
Subjects:
Age Factors
Animals
Electrophoresis, Polyacrylamide Gel
Female
Gene Expression Regulation, Developmental
Hypothyroidism - genetics
Hypothyroidism - metabolism
Isomerism
Life Sciences (General)
Muscle Development
Muscle Fibers, Skeletal - chemistry
Muscle Fibers, Skeletal - physiology
Muscle, Skeletal - chemistry
Muscle, Skeletal - cytology
Muscle, Skeletal - growth & development
Myosin Heavy Chains - chemistry
Myosin Heavy Chains - genetics
Polymorphism, Genetic
Pregnancy
Rats
Rats, Sprague-Dawley
Space life sciences
Thyroxine - blood
Triiodothyronine - blood
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Department of Physiology and Biophysics and Orthopaedics, College of Medicine, University of California, Irvine, California 92697 The primary objective of this study was to follow the developmental time course of myosin heavy chain (MHC) isoform transitions in single fibers of the rodent plantaris muscle. Hypothyroidism was used in conjunction with single-fiber analyses to better describe a possible linkage between the neonatal and fast type IIB MHC isoforms during development. In contrast to the general concept that developmental MHC isoform transitions give rise to muscle fibers that express only a single MHC isoform, the single-fiber analyses revealed a very high degree of MHC polymorphism throughout postnatal development. In the adult state, MHC polymorphism was so pervasive that the rodent plantaris muscles contained ~12-15 different pools of fibers (i.e., fiber types). The degree of polymorphism observed at the single-fiber level made it difficult to determine specific developmental schemes analogous to those observed previously for the rodent soleus muscle. However, hypothyroidism was useful in that it confirmed a possible link between the developmental regulation of the neonatal and fast type IIB MHC isoforms. myosin heavy chain isoforms; electrophoresis; neonatal; plantaris muscle
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.2000.278.4.R1099