Serum antibody responses of cats to soluble whole cell antigens of feline Porphyromonas gingivalis

• Published in: Veterinary microbiology 2000-04, Vol.73 (1), p.37-49
• Main Authors: Norris, Jacqueline M, Love, Daria N
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antibodies, Bacterial - blood; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacteroidaceae Infections - immunology; Bacteroidaceae Infections - microbiology; Bacteroidaceae Infections - veterinary; Blotting, Western - veterinary; Cat; Cat Diseases - immunology; Cat Diseases - microbiology; Cats; Electrophoresis, Polyacrylamide Gel - veterinary; Female; Immune response — cat; Male; Molecular Sequence Data; Periodontitis; Periodontitis - immunology; Periodontitis - microbiology; Periodontitis - veterinary; Porphyromonas gingivalis; Porphyromonas gingivalis - chemistry; Porphyromonas gingivalis - immunology; Proteinases; Sequence Alignment; Sequence Analysis, Protein; Sequence Homology, Amino Acid
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(00)00153-X

The whole cell soluble antigens of two strains (VPB 3457 and VPB 3492) of feline Porphyromonas gingivalis were analysed by Western blotting using serum taken from 40 domestic cats with various grades of periodontal disease. Five strongly immunogenic protein bands (70, 34, 27, 24 and 19 kDa) from VPB 3457 and seven from VPB 3492 (58, 44, 34, 27, 25, 24 and 21 kDa) were selected for further study. A significant positive correlation was found between the serum antibody response to the 70, 34, 27, 24 and 19 kDa bands of VPB 3457 and the 58, 44, 25, 24 and 21 kDa bands of VPB 3492 and the overall periodontal grade. A significant positive correlation was also found between the serum antibody response to the 24 kDa band of VPB 3457 and the total colony forming units of P. gingivalis. N-terminal sequencing of the 44 kDa band of VPB 3492 showed 75% identity with the translated amino acids from the hag A (haemagglutinin) gene of a human strain of P. gingivalis and N-terminal amino acid sequence of the 27 kDa band of VPB 3457 showed 88% identity with the amino acid sequences translated from DNA of purported genes coding for variously named proteinases of human strains of P. gingivalis.