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Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization
Dot blot hybridization and the polymerase chain reaction (PCR) were used to study aminoglycoside-modifying enzymes in aminoglycoside-resistant staphylococci isolated in hospitals in Kuwait. DNA encoding the acetyltransferase (AAC) (6′)–phosphotransferase (APH) (2′′), nucleotidyltransferase (ANT) (4′...
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Published in: | International journal of antimicrobial agents 2000-02, Vol.13 (4), p.273-279 |
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description | Dot blot hybridization and the polymerase chain reaction (PCR) were used to study aminoglycoside-modifying enzymes in aminoglycoside-resistant staphylococci isolated in hospitals in Kuwait. DNA encoding the acetyltransferase (AAC) (6′)–phosphotransferase (APH) (2′′), nucleotidyltransferase (ANT) (4′) and APH (3′) enzymes were detected in
Staphylococcus aureus and coagulase negative staphylococci. ANT (4′) was the most common enzyme detected. The majority of isolates contained genes for all three modifying enzymes, AAC (6′)–APH (2′′), ANT (4′) and APH (3′); only few isolates carried genes for a single modifying enzyme. Genes encoding the AAC (6′)–APH (2′′) were detected in all except two gentamicin-resistant isolates. In these isolates the genes for the AAC (6′)–APH (2′′) enzyme could not be detected by PCR and dot blot hybridization. Whereas antibiotic resistance testing could be used to predict the presence of the AAC (6′)–APH (2′′) enzyme it was not useful in predicting the presence of the ANT (4′) or APH (3′) enzymes in gentamicin-resistant isolates. Results obtained with dot blot hybridization were comparable to those obtained with PCR. However, PCR was fast and results were obtained within the same day. Therefore PCR would be preferred for the detection and confirmation of the presence of aminoglycoside-modifying enzymes in clinical microbiology laboratories. |
doi_str_mv | 10.1016/S0924-8579(99)00124-7 |
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Staphylococcus aureus and coagulase negative staphylococci. ANT (4′) was the most common enzyme detected. The majority of isolates contained genes for all three modifying enzymes, AAC (6′)–APH (2′′), ANT (4′) and APH (3′); only few isolates carried genes for a single modifying enzyme. Genes encoding the AAC (6′)–APH (2′′) were detected in all except two gentamicin-resistant isolates. In these isolates the genes for the AAC (6′)–APH (2′′) enzyme could not be detected by PCR and dot blot hybridization. Whereas antibiotic resistance testing could be used to predict the presence of the AAC (6′)–APH (2′′) enzyme it was not useful in predicting the presence of the ANT (4′) or APH (3′) enzymes in gentamicin-resistant isolates. Results obtained with dot blot hybridization were comparable to those obtained with PCR. However, PCR was fast and results were obtained within the same day. Therefore PCR would be preferred for the detection and confirmation of the presence of aminoglycoside-modifying enzymes in clinical microbiology laboratories.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/S0924-8579(99)00124-7</identifier><identifier>PMID: 10755241</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>6'-Phosphotransferase ; acetyltransferase ; Acetyltransferases - genetics ; Aminoglycosides ; Anti-Bacterial Agents - metabolism ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Biological and medical sciences ; Biotechnology ; Cross Infection - microbiology ; Dot blot hybridization ; Drug Resistance, Microbial ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic engineering ; Genetic technics ; Genotype ; Humans ; In vitro gene amplification. Pcr technique ; Kanamycin Kinase - genetics ; Medical sciences ; Methods. Procedures. Technologies ; Microbial Sensitivity Tests ; Nucleic Acid Hybridization ; nucleotidyltransferase ; Nucleotidyltransferases - genetics ; Pharmacology. Drug treatments ; Phosphotransferases (Alcohol Group Acceptor) - genetics ; Polymerase Chain Reaction ; Staphylococcal Infections - microbiology ; Staphylococcus - enzymology ; Staphylococcus - genetics ; Staphylococcus - isolation & purification ; Staphylococcus aureus</subject><ispartof>International journal of antimicrobial agents, 2000-02, Vol.13 (4), p.273-279</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-3d9bbeda5f93276791e28661279c1ecbd84631e3b4cabd63eed20ade5c31312e3</citedby><cites>FETCH-LOGICAL-c421t-3d9bbeda5f93276791e28661279c1ecbd84631e3b4cabd63eed20ade5c31312e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1266337$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10755241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Udo, E.E</creatorcontrib><creatorcontrib>Dashti, A.A</creatorcontrib><title>Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>Dot blot hybridization and the polymerase chain reaction (PCR) were used to study aminoglycoside-modifying enzymes in aminoglycoside-resistant staphylococci isolated in hospitals in Kuwait. DNA encoding the acetyltransferase (AAC) (6′)–phosphotransferase (APH) (2′′), nucleotidyltransferase (ANT) (4′) and APH (3′) enzymes were detected in
Staphylococcus aureus and coagulase negative staphylococci. ANT (4′) was the most common enzyme detected. The majority of isolates contained genes for all three modifying enzymes, AAC (6′)–APH (2′′), ANT (4′) and APH (3′); only few isolates carried genes for a single modifying enzyme. Genes encoding the AAC (6′)–APH (2′′) were detected in all except two gentamicin-resistant isolates. In these isolates the genes for the AAC (6′)–APH (2′′) enzyme could not be detected by PCR and dot blot hybridization. Whereas antibiotic resistance testing could be used to predict the presence of the AAC (6′)–APH (2′′) enzyme it was not useful in predicting the presence of the ANT (4′) or APH (3′) enzymes in gentamicin-resistant isolates. Results obtained with dot blot hybridization were comparable to those obtained with PCR. However, PCR was fast and results were obtained within the same day. Therefore PCR would be preferred for the detection and confirmation of the presence of aminoglycoside-modifying enzymes in clinical microbiology laboratories.</description><subject>6'-Phosphotransferase</subject><subject>acetyltransferase</subject><subject>Acetyltransferases - genetics</subject><subject>Aminoglycosides</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cross Infection - microbiology</subject><subject>Dot blot hybridization</subject><subject>Drug Resistance, Microbial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genotype</subject><subject>Humans</subject><subject>In vitro gene amplification. Pcr technique</subject><subject>Kanamycin Kinase - genetics</subject><subject>Medical sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial Sensitivity Tests</subject><subject>Nucleic Acid Hybridization</subject><subject>nucleotidyltransferase</subject><subject>Nucleotidyltransferases - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Phosphotransferases (Alcohol Group Acceptor) - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus - enzymology</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - isolation & purification</subject><subject>Staphylococcus aureus</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAQgC0EotvCTwD5gFA5BPxI4vhUobY8pEocgLPlx2TXKLEXO4uU3vnfOM2K9taLLc98M2P7Q-gVJe8poe2H70SyuuoaIc-lfEcILSfxBG1oJ1glJOVP0eY_coJOc_5VoIbXzXN0QoloGlbTDfp7BRPYyceAY4-3ECBjCDY6H7ZYjz7E7TDbmL2DaizRfl4SEG7nsZA-4Dzp_W4eoo3WemxmvI9DySWdAdudLkQCvQ7QwWEXJ2yGsuxmk7zzt3pJvUDPej1keHncz9DPT9c_Lr9UN98-f738eFPZmtGp4k4aA043veRMtOWVwLq2pUxIS8Ea19Utp8BNbbVxLQdwjGgHjeWUUwb8DL1d--5T_H2APKnRZwvDoAPEQ1aCEsKJkI-CVNQdI4QVsFlBm2LOCXq1T37UaVaUqEWUuhOlFgtKSnUnSolS9_o44GBGcA-qVjMFeHMEdLZ66JMO1ud7jrUt50ufixWD8m1_PCSVrS8CwflUvCoX_SM3-QdwvLMD</recordid><startdate>200002</startdate><enddate>200002</enddate><creator>Udo, E.E</creator><creator>Dashti, A.A</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200002</creationdate><title>Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization</title><author>Udo, E.E ; Dashti, A.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-3d9bbeda5f93276791e28661279c1ecbd84631e3b4cabd63eed20ade5c31312e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>6'-Phosphotransferase</topic><topic>acetyltransferase</topic><topic>Acetyltransferases - genetics</topic><topic>Aminoglycosides</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Antibacterial agents</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cross Infection - microbiology</topic><topic>Dot blot hybridization</topic><topic>Drug Resistance, Microbial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genotype</topic><topic>Humans</topic><topic>In vitro gene amplification. Pcr technique</topic><topic>Kanamycin Kinase - genetics</topic><topic>Medical sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial Sensitivity Tests</topic><topic>Nucleic Acid Hybridization</topic><topic>nucleotidyltransferase</topic><topic>Nucleotidyltransferases - genetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Phosphotransferases (Alcohol Group Acceptor) - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus - enzymology</topic><topic>Staphylococcus - genetics</topic><topic>Staphylococcus - isolation & purification</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Udo, E.E</creatorcontrib><creatorcontrib>Dashti, A.A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Udo, E.E</au><au>Dashti, A.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2000-02</date><risdate>2000</risdate><volume>13</volume><issue>4</issue><spage>273</spage><epage>279</epage><pages>273-279</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>Dot blot hybridization and the polymerase chain reaction (PCR) were used to study aminoglycoside-modifying enzymes in aminoglycoside-resistant staphylococci isolated in hospitals in Kuwait. DNA encoding the acetyltransferase (AAC) (6′)–phosphotransferase (APH) (2′′), nucleotidyltransferase (ANT) (4′) and APH (3′) enzymes were detected in
Staphylococcus aureus and coagulase negative staphylococci. ANT (4′) was the most common enzyme detected. The majority of isolates contained genes for all three modifying enzymes, AAC (6′)–APH (2′′), ANT (4′) and APH (3′); only few isolates carried genes for a single modifying enzyme. Genes encoding the AAC (6′)–APH (2′′) were detected in all except two gentamicin-resistant isolates. In these isolates the genes for the AAC (6′)–APH (2′′) enzyme could not be detected by PCR and dot blot hybridization. Whereas antibiotic resistance testing could be used to predict the presence of the AAC (6′)–APH (2′′) enzyme it was not useful in predicting the presence of the ANT (4′) or APH (3′) enzymes in gentamicin-resistant isolates. Results obtained with dot blot hybridization were comparable to those obtained with PCR. However, PCR was fast and results were obtained within the same day. Therefore PCR would be preferred for the detection and confirmation of the presence of aminoglycoside-modifying enzymes in clinical microbiology laboratories.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>10755241</pmid><doi>10.1016/S0924-8579(99)00124-7</doi><tpages>7</tpages></addata></record> |
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subjects | 6'-Phosphotransferase acetyltransferase Acetyltransferases - genetics Aminoglycosides Anti-Bacterial Agents - metabolism Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Biotechnology Cross Infection - microbiology Dot blot hybridization Drug Resistance, Microbial Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic technics Genotype Humans In vitro gene amplification. Pcr technique Kanamycin Kinase - genetics Medical sciences Methods. Procedures. Technologies Microbial Sensitivity Tests Nucleic Acid Hybridization nucleotidyltransferase Nucleotidyltransferases - genetics Pharmacology. Drug treatments Phosphotransferases (Alcohol Group Acceptor) - genetics Polymerase Chain Reaction Staphylococcal Infections - microbiology Staphylococcus - enzymology Staphylococcus - genetics Staphylococcus - isolation & purification Staphylococcus aureus |
title | Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization |
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