Consequences of Inspired Oxygen Fraction Manipulation on Myocardial Oxygen Pressure, Adenosine and Lactate Concentrations: A Combined Myocardial Microdialysis and Sensitive Oxygen Electrode Study in Pigs

• Published in: Journal of molecular and cellular cardiology 2000-03, Vol.32 (3), p.493-504
• Main Authors: Siaghy, E.M, Devaux, Y, Sfaksi, N, Carteaux, J.P, Ungureanu-Longrois, D, Zannad, F, Villemot, J.P, Burlet, C, Mertes, P.M
• Format: Article
• Language: English
• Subjects: Adenosine; Adenosine - metabolism; Animals; Blood Gas Analysis; Cardiac microdialysis; Electrodes; Glucose - metabolism; Hemodynamics; Hypoxia; Lactates - metabolism; Microdialysis; Myocardial oxygenation; Myocardium - metabolism; Oxygen - metabolism; PtiO2; Swine
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1006/jmcc.1999.1094

Adenosine is a potent vasodilator whose concentration has been shown to increase in cardiac tissue in response to hypoxia. However, the time-dependent relationship between the levels of myocardial interstitial adenosine and tissue oxygenation has not yet been completely established. Therefore, the purpose of this study was to investigate the complex relationship between tissue myocardial oxygen tension (PtiO2) and interstitial myocardial adenosine and lactate concentrations by developing a new technique which combines a cardiac microdialysis probe and a Clark-type PO2 electrode. The combined and the single microdialysis probes were implanted in the left ventricular myocardium of anesthetized pigs. The consequences of the combined use of microdialysis and P O2probes on myocardial PtiO2and microdialysis performances against glucose were evaluated. A moderate but significant reduction in the relative recovery against glucose of the combined probe was observed when compared to that of the single microdialysis probe (42±2v 32±1%, mean±S.E.M.n=5 P