Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation

• Published in: Biotechnology and bioengineering 2000-05, Vol.68 (4), p.466-470
• Main Authors: Lee, Sang Yup, Wong, Heng Ho, Choi, Jong-il, Lee, Seung Hwan, Lee, Sang Cheol, Han, Chul Soo
• Format: Article
• Language: English
• Subjects: Animal cell culture; Bacteria; Batch cell culture; Biological and medical sciences; Bioreactors; Biotechnology; Carbon; Carboxylic acids; Culture Media; Fundamental and applied biological sciences. Psychology; high-cell-density culture; medium-chain-length polyhydroxyalkanoates; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Oleic acid; Optimization; Phosphorus; Phosphorus - metabolism; Polyesters - metabolism; Potassium compounds; Pseudomonas putida; Pseudomonas putida - growth & development; Pseudomonas putida - metabolism
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/(SICI)1097-0290(20000520)68:4<466::AID-BIT12>3.0.CO;2-T

High‐cell‐density fed‐batch cultures of Pseudomonas putida were carried out for the production of medium‐chain‐length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, a high cell concentration of 173 g/L was achieved, but the PHA concentration and PHA content were only 32.3 g/L and 18.7 wt%, respectively. To increase the PHA concentration and content, phosphorus limitation was applied during fed‐bath culture by reducing the initial KH2PO4 concentration. When the initial KH2PO4 concentration was reduced to 4 g/L, cell concentration, PHA concentration, and PHA content obtained in 38 h were 141 g/L, 72.6 g/L, and 51.4 wt%, respectively, resulting in a high productivity of 1.91 g PHA/L per hour. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 466–470, 2000.