Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein

• Published in: Vaccine 2000-05, Vol.18 (22), p.2426-2434
• Main Authors: Raviprakash, K., Kochel, T.J., Ewing, D., Simmons, M., Phillips, I., Hayes, C.G., Porter, K.R.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - biosynthesis; Antibody response; Base Sequence; Biological and medical sciences; Cell Line; Dengue - immunology; Dengue - prevention & control; Dengue virus; Dengue Virus - genetics; Dengue Virus - immunology; DNA Primers - genetics; DNA vaccine; Fundamental and applied biological sciences. Psychology; Humans; Immunization; Mice; Mice, Inbred BALB C; Microbiology; Mouse; Neutralization Tests; Peptide Fragments - genetics; Peptide Fragments - immunology; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Vaccines - genetics; Viral Vaccines - immunology; Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(99)00570-8

Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E.