Validation of equilibration and chromium reduction methods for deuterium measurements of fluid volumes

• Published in: Journal of applied physiology (1985) 2001-08, Vol.91 (2), p.733-736
• Main Authors: Hedestig, T, Ebberyd, A, Lindahl, S. G. E
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Body fluids; Body Fluids - chemistry; Body Fluids - physiology; Body Water - chemistry; Body Water - physiology; Chromium; Deuterium; Fundamental and applied biological sciences. Psychology; General aspects; Humans; Isotopes; Mass Spectrometry - methods; Medicin och hälsovetenskap; Oxidation-Reduction; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity; Shock - physiopathology; Spectrum analysis; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Wounds and Injuries - physiopathology
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.2001.91.2.733

Department of Anaesthesia and Intensive Care, Karolinska Hospital and Institute, S-171 76 Stockholm, Sweden Determinations of fluid volumes are of importance for correct treatment of patients subjected to shock and trauma. Gas isotope ratio mass spectrometry (GIRMS) is an advanced method for analysis of stable isotopes. These can be used as tracers for measurement of various fluid volumes. In the current in vitro study, deuterium was used to determine different volumes of water simulating a range of body fluid volumes from neonates to adults. A high-precision scale gave control weights (i.e., volumes), and two methods, equilibration (EQ) and chromium reduction (CR), were compared by use of a GIRMS. The coefficient of variation was