Quantitation of in vivo measurements with carbon fiber microelectrodes

Fast-scan cyclic voltammetry (FSCV) at carbon fiber disk microelectrodes and quantitative microdialysis were used to measure striatal concentration changes of N-acetyl- p-aminophenol (APAP, acetaminophen) following an intraperitoneal injection of 75 mg/kg APAP in rats. The goal of this work was to d...

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Bibliographic Details
Published in:Journal of neuroscience methods 2000-02, Vol.95 (2), p.95-102
Main Authors: Logman, Michael J, Budygin, Evgeny A, Gainetdinov, Raul R, Wightman, R.Mark
Format: Article
Language:English
Subjects:
Acetaminophen
Acetaminophen - pharmacokinetics
Analgesics, Non-Narcotic - pharmacokinetics
Animals
Biological and medical sciences
Calibration - standards
Carbon Compounds, Inorganic
Carbon fiber microelectrodes
Fast-scan cyclic voltammetry
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
In Vitro Techniques
Male
Microdialysis
Microdialysis - methods
Microelectrodes - standards
Neostriatum - drug effects
Neostriatum - metabolism
Rats
Rats, Sprague-Dawley
Striatum
Vertebrates: nervous system and sense organs
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Summary:Fast-scan cyclic voltammetry (FSCV) at carbon fiber disk microelectrodes and quantitative microdialysis were used to measure striatal concentration changes of N-acetyl- p-aminophenol (APAP, acetaminophen) following an intraperitoneal injection of 75 mg/kg APAP in rats. The goal of this work was to determine which in vitro calibration procedure, precalibration or postcalibration, gave the most accurate results when using carbon fiber microelectrodes in vivo. Voltammetric detection of APAP in vivo was complicated with normal electrodes by interference from pH changes. An electrode treatment was used to minimize electrode sensitivity to pH and this allowed successful APAP detection. In vitro calibrations of the treated carbon fiber disk microelectrodes before and after the in vivo experiment were used to calculate APAP concentration changes measured in vivo and compared to microdialysis results. The maximal striatal APAP concentration determined by microdialysis, adjusted for in vitro recovery, was 23.1 μM. The electrochemical results were approximately two times greater (postcalibration) or smaller (precalibration) than the microdialysis result.
ISSN:0165-0270
1872-678X
DOI:10.1016/S0165-0270(99)00155-7