Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method

We have used a new single‐step real‐time reverse transcription polymerase chain reaction (RT‐PCR) method to quantify BCR‐ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR‐ABL expression was...

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Bibliographic Details
Published in:British journal of haematology 2001-06, Vol.113 (4), p.1072-1075
Main Authors: Elmaagacli, Ahmet H., Freist, Annette, Hahn, Meinhard, Opalka, Bertram, Seeber, Siegfried, Schaefer, Ulrich W., Beelen, D. W.
Format: Article
Language:English
Subjects:
Adolescent
Adult
allogeneic transplantation
BCR‐ABL
Biological and medical sciences
CML
Cytogenetic Analysis
Female
Fusion Proteins, bcr-abl - genetics
Hematologic and hematopoietic diseases
Hematology
Hematopoietic Stem Cell Transplantation
Humans
In Situ Hybridization, Fluorescence
Investigative techniques, diagnostic techniques (general aspects)
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - diagnosis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - surgery
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
Middle Aged
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymerase Chain Reaction - methods
real‐time PCR
Recurrence
relapse
Transplantation, Homologous
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Summary:We have used a new single‐step real‐time reverse transcription polymerase chain reaction (RT‐PCR) method to quantify BCR‐ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR‐ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real‐time RT‐PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7·5 pg. The median normalized BCR‐ABL amount differed significantly (P 
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2001.02858.x