Transient up-regulation of liver mitochondrial thymidine kinase activity in proliferating mitochondria

Administration of the fatty acid analogue tetradecylthioacetic acid (TTA) to rodents up-regulates peroxisomal and mitochondrial lipid-metabolizing enzymes and induces a proliferation of these organelles in hepatocytes. We show here that male NMRI mice fed a diet containing 0.3% (w/w) TTA revealed a...

Full description

Saved in:
Bibliographic Details
Published in:IUBMB life 2001-02, Vol.51 (2), p.99-104
Main Authors: Elholm, M, Hollås, H, Issalene, C, Barroso, J F, Berge, R K, Flatmark, T
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Body Weight - drug effects
DNA Polymerase gamma
DNA Primers
DNA Replication
DNA, Mitochondrial - biosynthesis
DNA-Directed DNA Polymerase - metabolism
Male
Mice
Mitochondria, Liver - drug effects
Mitochondria, Liver - enzymology
Mitochondria, Liver - physiology
Organ Size - drug effects
Sulfides - pharmacology
Thymidine Kinase - metabolism
Up-Regulation
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Administration of the fatty acid analogue tetradecylthioacetic acid (TTA) to rodents up-regulates peroxisomal and mitochondrial lipid-metabolizing enzymes and induces a proliferation of these organelles in hepatocytes. We show here that male NMRI mice fed a diet containing 0.3% (w/w) TTA revealed a transient two-fold increase in the incorporation of [3H]thymidine into the liver mtDNA followed by a 1.6-fold increase in the content of mtDNA. In addition, a transient three-fold increase in the mitochondrial thymidine kinase (TK2) activity and a slight increase in the DNA polymerase gamma activity was observed, indicating that the TTA induced mitochondrial proliferation is linked to an up-regulation of the mitochondrial thymidine kinase activity.
ISSN:1521-6543
1521-6551
DOI:10.1080/15216540152122094